Cytofluorometric quantification of cell death elicited by NLR proteins

Valentina Sica, Gwenola Manic, Guido Kroemer, Ilio Vitale, Lorenzo Galluzzi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-245
Number of pages15
Volume1417
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1417
ISSN (Print)10643745

Keywords

  • Annexin A5
  • Apoptosis
  • Caspase
  • Mitochondria
  • Necrosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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