Cytogenetic analysis of hemopoietic peripheral blood cells collected by leukapheresis after intensive chemotherapy in advanced phase Philadelphia-positive chronic myelogenous leukemia

Mario Sessarego, Giuseppina Fugazza, Francesco Frassoni, Raffaella Defferrari, Roberto Bruzzone, A. Michele Carella

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peripheral hemopoietic blood cells previously collected by leukapheresis were reinfused in advanced phase Philadelphia (Ph)-positive chronic myelogenous leukemic patients to promote the recovery of bone marrow function after intensive radiochemotherapy. Cytogenetlc analysis was performed on these cells, Induced to proliferate and to be mobilized by a first administration of marrow toxic drugs and collected when the white blood cell count was very low. In five patients only Ph-negative apparently normal cells were found. In five cases different proportions of Ph+/Ph- cells were observed and in the remaining five cases only Ph+ cells were present. Chromosomal abnormalities other than Ph, not detected in the cytogenetic analysis performed on bone marrow cells before chemotherapy treatment, were found in five cases. These findings confirm that Ph- cells can persist in the marrow of Ph+ patients in the advanced phase of disease and Indicate that a high percentage of leukemic cells retain karyotype evolution not detectable using standard drawing and culture techniques.

Original languageEnglish
Pages (from-to)715-719
Number of pages5
JournalLeukemia
Volume6
Issue number7
Publication statusPublished - Jul 1992

Fingerprint

Leukapheresis
Cytogenetic Analysis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Blood Cells
Drug Therapy
Bone Marrow
Culture Techniques
Poisons
Chemoradiotherapy
Karyotype
Leukocyte Count
Chromosome Aberrations
Bone Marrow Cells

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Cytogenetic analysis of hemopoietic peripheral blood cells collected by leukapheresis after intensive chemotherapy in advanced phase Philadelphia-positive chronic myelogenous leukemia. / Sessarego, Mario; Fugazza, Giuseppina; Frassoni, Francesco; Defferrari, Raffaella; Bruzzone, Roberto; Carella, A. Michele.

In: Leukemia, Vol. 6, No. 7, 07.1992, p. 715-719.

Research output: Contribution to journalArticle

@article{91234260a78b4729a615e3bff89b46ca,
title = "Cytogenetic analysis of hemopoietic peripheral blood cells collected by leukapheresis after intensive chemotherapy in advanced phase Philadelphia-positive chronic myelogenous leukemia",
abstract = "Peripheral hemopoietic blood cells previously collected by leukapheresis were reinfused in advanced phase Philadelphia (Ph)-positive chronic myelogenous leukemic patients to promote the recovery of bone marrow function after intensive radiochemotherapy. Cytogenetlc analysis was performed on these cells, Induced to proliferate and to be mobilized by a first administration of marrow toxic drugs and collected when the white blood cell count was very low. In five patients only Ph-negative apparently normal cells were found. In five cases different proportions of Ph+/Ph- cells were observed and in the remaining five cases only Ph+ cells were present. Chromosomal abnormalities other than Ph, not detected in the cytogenetic analysis performed on bone marrow cells before chemotherapy treatment, were found in five cases. These findings confirm that Ph- cells can persist in the marrow of Ph+ patients in the advanced phase of disease and Indicate that a high percentage of leukemic cells retain karyotype evolution not detectable using standard drawing and culture techniques.",
author = "Mario Sessarego and Giuseppina Fugazza and Francesco Frassoni and Raffaella Defferrari and Roberto Bruzzone and Carella, {A. Michele}",
year = "1992",
month = "7",
language = "English",
volume = "6",
pages = "715--719",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Cytogenetic analysis of hemopoietic peripheral blood cells collected by leukapheresis after intensive chemotherapy in advanced phase Philadelphia-positive chronic myelogenous leukemia

AU - Sessarego, Mario

AU - Fugazza, Giuseppina

AU - Frassoni, Francesco

AU - Defferrari, Raffaella

AU - Bruzzone, Roberto

AU - Carella, A. Michele

PY - 1992/7

Y1 - 1992/7

N2 - Peripheral hemopoietic blood cells previously collected by leukapheresis were reinfused in advanced phase Philadelphia (Ph)-positive chronic myelogenous leukemic patients to promote the recovery of bone marrow function after intensive radiochemotherapy. Cytogenetlc analysis was performed on these cells, Induced to proliferate and to be mobilized by a first administration of marrow toxic drugs and collected when the white blood cell count was very low. In five patients only Ph-negative apparently normal cells were found. In five cases different proportions of Ph+/Ph- cells were observed and in the remaining five cases only Ph+ cells were present. Chromosomal abnormalities other than Ph, not detected in the cytogenetic analysis performed on bone marrow cells before chemotherapy treatment, were found in five cases. These findings confirm that Ph- cells can persist in the marrow of Ph+ patients in the advanced phase of disease and Indicate that a high percentage of leukemic cells retain karyotype evolution not detectable using standard drawing and culture techniques.

AB - Peripheral hemopoietic blood cells previously collected by leukapheresis were reinfused in advanced phase Philadelphia (Ph)-positive chronic myelogenous leukemic patients to promote the recovery of bone marrow function after intensive radiochemotherapy. Cytogenetlc analysis was performed on these cells, Induced to proliferate and to be mobilized by a first administration of marrow toxic drugs and collected when the white blood cell count was very low. In five patients only Ph-negative apparently normal cells were found. In five cases different proportions of Ph+/Ph- cells were observed and in the remaining five cases only Ph+ cells were present. Chromosomal abnormalities other than Ph, not detected in the cytogenetic analysis performed on bone marrow cells before chemotherapy treatment, were found in five cases. These findings confirm that Ph- cells can persist in the marrow of Ph+ patients in the advanced phase of disease and Indicate that a high percentage of leukemic cells retain karyotype evolution not detectable using standard drawing and culture techniques.

UR - http://www.scopus.com/inward/record.url?scp=0026755446&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026755446&partnerID=8YFLogxK

M3 - Article

C2 - 1625491

AN - SCOPUS:0026755446

VL - 6

SP - 715

EP - 719

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 7

ER -