Cytogenetic analysis of human cells reveals specific patterns of DNA damage in replicative and oncogene-induced senescence

Germana Falcone, Alessia Mazzola, Flavia Michelini, Gianluca Bossi, Federica Censi, Maria G. Biferi, Luisa Minghetti, Giovanna Floridia, Maurizio Federico, Antonio Musio, Marco Crescenzi

Research output: Contribution to journalArticlepeer-review

Abstract

Senescence is thought to be triggered by DNA damage, usually indirectly assessed as activation of the DNA damage response (DDR), but direct surveys of genetic damage are lacking. Here, we mitotically reactivate senescent human fibroblasts to evaluate their cytogenetic damage. We show that replicative senescence is generally characterized by telomeric fusions. However, both telomeric and extratelomeric aberrations are prevented by hTERT, indicating that even non-telomeric damage descends from the lack of telomerase. Compared with replicative senescent cells, oncogene-induced senescent fibroblasts display significantly higher levels of DNA damage, depicting how oncogene activation can catalyze the generation of further, potentially tumorigenic, genetic damage.

Original languageEnglish
Pages (from-to)312-315
Number of pages4
JournalAging Cell
Volume12
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • Cell cycle inhibitors
  • Cytogenetic analysis
  • DNA damage
  • Oncogene-induced senescence
  • Replicative senescence
  • Telomerase

ASJC Scopus subject areas

  • Cell Biology
  • Ageing

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