Cytogenetic and molecular study of two human neuroblastoma cell lines

Emilio Donti; Letizia Longo; Gian Paolo Tonini; Giovanna Verdona; Armando Melodia; Edoardo Lanino; Paolo Cornaglia-Ferraris

doi:10.1016/0165-4608(88)90188-4

Cytogenetic and molecular study of two human neuroblastoma cell lines

Emilio Donti, Letizia Longo, Gian Paolo Tonini, Giovanna Verdona, Armando Melodia, Edoardo Lanino, Paolo Cornaglia-Ferraris

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article

Abstract

Long-term cell cultures (GI-LA-N and GI-ME-N) were established from the metastases of two disseminated neuroblastomas (NB). The first was obtained from a lymph node biopsy of a stage III NB after 7 months of chemotherapy, and the second from a bone marrow specimen of a stage IV NB after 6 months of chemotherapy. Cytogenetic investigation revealed several structural and numerical alterations in both cell cultures, but the only common chromosomal aberration was partial monosomy of 1p. Moreover, at the 5th in vitro passage, GI-LA-N displayed a high number of double minutes, not seen in GI-ME-N even after 33 subcultures. Molecular analysis revealed N-myc oncogene amplification in GI-LA-N cells, whereas, only one copy was found in GI-ME-N. No structural N-myc rearrangement was detected in either cell culture.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Cancer Genetics and Cytogenetics
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/0165-4608(88)90188-4
Publication status	Published - 1988

ASJC Scopus subject areas

Cancer Research
Genetics
Molecular Biology

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Donti, E., Longo, L., Tonini, G. P., Verdona, G., Melodia, A., Lanino, E., & Cornaglia-Ferraris, P. (1988). Cytogenetic and molecular study of two human neuroblastoma cell lines. Cancer Genetics and Cytogenetics, 30(2), 225-231. https://doi.org/10.1016/0165-4608(88)90188-4

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title = "Cytogenetic and molecular study of two human neuroblastoma cell lines",

abstract = "Long-term cell cultures (GI-LA-N and GI-ME-N) were established from the metastases of two disseminated neuroblastomas (NB). The first was obtained from a lymph node biopsy of a stage III NB after 7 months of chemotherapy, and the second from a bone marrow specimen of a stage IV NB after 6 months of chemotherapy. Cytogenetic investigation revealed several structural and numerical alterations in both cell cultures, but the only common chromosomal aberration was partial monosomy of 1p. Moreover, at the 5th in vitro passage, GI-LA-N displayed a high number of double minutes, not seen in GI-ME-N even after 33 subcultures. Molecular analysis revealed N-myc oncogene amplification in GI-LA-N cells, whereas, only one copy was found in GI-ME-N. No structural N-myc rearrangement was detected in either cell culture.",

author = "Emilio Donti and Letizia Longo and Tonini, {Gian Paolo} and Giovanna Verdona and Armando Melodia and Edoardo Lanino and Paolo Cornaglia-Ferraris",

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AU - Donti, Emilio

AU - Longo, Letizia

AU - Tonini, Gian Paolo

AU - Verdona, Giovanna

AU - Melodia, Armando

AU - Lanino, Edoardo

AU - Cornaglia-Ferraris, Paolo

PY - 1988

Y1 - 1988

N2 - Long-term cell cultures (GI-LA-N and GI-ME-N) were established from the metastases of two disseminated neuroblastomas (NB). The first was obtained from a lymph node biopsy of a stage III NB after 7 months of chemotherapy, and the second from a bone marrow specimen of a stage IV NB after 6 months of chemotherapy. Cytogenetic investigation revealed several structural and numerical alterations in both cell cultures, but the only common chromosomal aberration was partial monosomy of 1p. Moreover, at the 5th in vitro passage, GI-LA-N displayed a high number of double minutes, not seen in GI-ME-N even after 33 subcultures. Molecular analysis revealed N-myc oncogene amplification in GI-LA-N cells, whereas, only one copy was found in GI-ME-N. No structural N-myc rearrangement was detected in either cell culture.

AB - Long-term cell cultures (GI-LA-N and GI-ME-N) were established from the metastases of two disseminated neuroblastomas (NB). The first was obtained from a lymph node biopsy of a stage III NB after 7 months of chemotherapy, and the second from a bone marrow specimen of a stage IV NB after 6 months of chemotherapy. Cytogenetic investigation revealed several structural and numerical alterations in both cell cultures, but the only common chromosomal aberration was partial monosomy of 1p. Moreover, at the 5th in vitro passage, GI-LA-N displayed a high number of double minutes, not seen in GI-ME-N even after 33 subcultures. Molecular analysis revealed N-myc oncogene amplification in GI-LA-N cells, whereas, only one copy was found in GI-ME-N. No structural N-myc rearrangement was detected in either cell culture.

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