Cytogenetic and molecular study of two human neuroblastoma cell lines

Emilio Donti, Letizia Longo, Gian Paolo Tonini, Giovanna Verdona, Armando Melodia, Edoardo Lanino, Paolo Cornaglia-Ferraris

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Long-term cell cultures (GI-LA-N and GI-ME-N) were established from the metastases of two disseminated neuroblastomas (NB). The first was obtained from a lymph node biopsy of a stage III NB after 7 months of chemotherapy, and the second from a bone marrow specimen of a stage IV NB after 6 months of chemotherapy. Cytogenetic investigation revealed several structural and numerical alterations in both cell cultures, but the only common chromosomal aberration was partial monosomy of 1p. Moreover, at the 5th in vitro passage, GI-LA-N displayed a high number of double minutes, not seen in GI-ME-N even after 33 subcultures. Molecular analysis revealed N-myc oncogene amplification in GI-LA-N cells, whereas, only one copy was found in GI-ME-N. No structural N-myc rearrangement was detected in either cell culture.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalCancer Genetics and Cytogenetics
Issue number2
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology


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