Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines: Comparison between two- and three-dimensional cell culture systems

Leda Roncoroni, Luca Elli, Maria Teresa Bardella, Elena Dogliotti, Maria Grazia Grimoldi, Silvia Viaggi, Eugenio Erba, Annalisa Zunino, Luisa Doneda

Research output: Contribution to journalArticle

Abstract

The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalCancer Investigation
Volume28
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • Cell cycle
  • CGH
  • Colon carcinoma
  • Cytogenetic analysis
  • Spheroids

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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