TY - JOUR
T1 - Cytogenetic damage induced in human lymphocytes by four vanadium compounds and micronucleus analysis by fluorescence in situ hybridization with a centromeric probe
AU - Migliore, L.
AU - Bocciardi, R.
AU - Macrì, C.
AU - Lo Jacono, F.
PY - 1993
Y1 - 1993
N2 - The genotoxicity of four vanadium compounds, sodium metavanadate (NaVO3), ammonium metavanadate (NH4VO3), sodium ortovanadate (Na3VO4) and vanadyl sulfate (SVO5), was evaluated in human lymphocyte cultures using structural and numerical chromosome aberrations, micronuclei, sister-chromatid exchanges and satellite chromosome associations as endpoints. These compounds were not found to increase the frequency of structural chromosome aberrations whereas a significant increase in numerical aberrations, micronuclei and satellite associations was found. Since these results could have been related to a possible mechanism of the action of vanadium as a mitotic spindle poison, the fluorescence in situ hybridization (FISH) technique was applied to the human lymphocyte micronucleus assay, by means of an alphoid centromere-specific DNA probe. The four vanadium salts showed a micronucleus percentage with positive signal (presence of centromere and thus of whole chromosome(s)) that was always higher than 68% at all doses tested. That confirmed the aneuploidogenic potentiality of vanadium.
AB - The genotoxicity of four vanadium compounds, sodium metavanadate (NaVO3), ammonium metavanadate (NH4VO3), sodium ortovanadate (Na3VO4) and vanadyl sulfate (SVO5), was evaluated in human lymphocyte cultures using structural and numerical chromosome aberrations, micronuclei, sister-chromatid exchanges and satellite chromosome associations as endpoints. These compounds were not found to increase the frequency of structural chromosome aberrations whereas a significant increase in numerical aberrations, micronuclei and satellite associations was found. Since these results could have been related to a possible mechanism of the action of vanadium as a mitotic spindle poison, the fluorescence in situ hybridization (FISH) technique was applied to the human lymphocyte micronucleus assay, by means of an alphoid centromere-specific DNA probe. The four vanadium salts showed a micronucleus percentage with positive signal (presence of centromere and thus of whole chromosome(s)) that was always higher than 68% at all doses tested. That confirmed the aneuploidogenic potentiality of vanadium.
KW - Cytogenetic damage
KW - FISH technique
KW - Micronuclei
KW - Vanadium compounds
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U2 - 10.1016/0165-1218(93)90080-W
DO - 10.1016/0165-1218(93)90080-W
M3 - Article
C2 - 7694142
AN - SCOPUS:0027141611
VL - 319
SP - 205
EP - 213
JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
SN - 0165-1218
IS - 3
ER -