Cytogenetic, molecular and phenotypic characterization of the newly established renal carcinoma cell line KJ29. Evidence of translocations for chromosomes 1 and 3

C. Barletta, A. Bartolazzi, G. C. Reale, R. Gambari, C. Nastruzzi, R. Barbieri, L. Del Senno, A. Castagnoli, P. G. Natali

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The established non papillary human renal carcinoma cell line (RCC) KJ29 was submitted to a multiparametric characterization to evaluate its potential use for in vitro and in vivo studies. The cell line grows in vitro as monolayer as well as cell suspension. Cytogenetic analysis has shown a modal chromosome number of 50 with some marker chromosomes, including rearrangements of chromosomes 1 and 3. The antigenic phenotype is characterized by co-expression of cytokeratin and vimentin, as well as expression of urothelium differentiation antigens, low levels of class II MHC antigens and no class I antigens. A differential expression of the VLA-3 integrin heterodimer has been detected between the adherent and non adherent cell population. The cell line which is highly tumorigenic in athymic mice displays expression of erb B-2 and c-met oncogenes and high expression of cell-cycle related and Ha-ras 1 genes.

Original languageEnglish
Pages (from-to)2129-2136
Number of pages8
JournalAnticancer Research
Volume15
Issue number5 B
Publication statusPublished - 1995

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Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 1
Cytogenetics
Carcinoma
Kidney
Cell Line
Integrin alpha3beta1
Urothelium
Histocompatibility Antigens Class I
ras Genes
Cytogenetic Analysis
Histocompatibility Antigens Class II
Differentiation Antigens
Vimentin
Keratins
Genetic Markers
Oncogenes
Nude Mice
Integrins
Suspensions

Keywords

  • Integrins
  • Oncogenes
  • Renal carcinoma
  • Translocation of chromosomes 1 and 3
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Barletta, C., Bartolazzi, A., Reale, G. C., Gambari, R., Nastruzzi, C., Barbieri, R., ... Natali, P. G. (1995). Cytogenetic, molecular and phenotypic characterization of the newly established renal carcinoma cell line KJ29. Evidence of translocations for chromosomes 1 and 3. Anticancer Research, 15(5 B), 2129-2136.

Cytogenetic, molecular and phenotypic characterization of the newly established renal carcinoma cell line KJ29. Evidence of translocations for chromosomes 1 and 3. / Barletta, C.; Bartolazzi, A.; Reale, G. C.; Gambari, R.; Nastruzzi, C.; Barbieri, R.; Del Senno, L.; Castagnoli, A.; Natali, P. G.

In: Anticancer Research, Vol. 15, No. 5 B, 1995, p. 2129-2136.

Research output: Contribution to journalArticle

Barletta, C, Bartolazzi, A, Reale, GC, Gambari, R, Nastruzzi, C, Barbieri, R, Del Senno, L, Castagnoli, A & Natali, PG 1995, 'Cytogenetic, molecular and phenotypic characterization of the newly established renal carcinoma cell line KJ29. Evidence of translocations for chromosomes 1 and 3', Anticancer Research, vol. 15, no. 5 B, pp. 2129-2136.
Barletta, C. ; Bartolazzi, A. ; Reale, G. C. ; Gambari, R. ; Nastruzzi, C. ; Barbieri, R. ; Del Senno, L. ; Castagnoli, A. ; Natali, P. G. / Cytogenetic, molecular and phenotypic characterization of the newly established renal carcinoma cell line KJ29. Evidence of translocations for chromosomes 1 and 3. In: Anticancer Research. 1995 ; Vol. 15, No. 5 B. pp. 2129-2136.
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