Cytokine-activated bronchial epithelial cell pro-inflammatory functions are effectively downregulated in vitro by ciclesonide

M. Silvestri; L. Serpero; L. Petecchia; F. Sabatini; F. Cerasoli; G. A. Rossi

doi:10.1016/j.pupt.2005.05.006

Cytokine-activated bronchial epithelial cell pro-inflammatory functions are effectively downregulated in vitro by ciclesonide

M. Silvestri, L. Serpero, L. Petecchia, F. Sabatini, F. Cerasoli, G. A. Rossi

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

Ciclesonide, a new inhaled corticosteroid, is administered as a parent compound and converted in the airway mucosa into the active metabolite, desisobutyryl-(des-)ciclesonide. A study was designed to evaluate the ability of ciclesonide to modulate pro-inflammatory functions of human bronchial epithelial cell (HBEC) primary cultures being converted into des-ciclesonide. HBECs were stimulated with interleukin (IL)-4 and tumour necrosis factor (TNF)-α (20 ng/mL) in the presence of ciclesonide and intercellular adhesion molecule (ICAM)-1 expression, granulocyte-macrophage colony stimulating factor (GM-CSF) and IL-8 release evaluated respectively by FACS and ELISA. Ciclesonide (3 μM) significantly inhibited ICAM-1 expression by stimulated HBECs, already after 3 h and still after 48 h culture (p

Original language	English
Pages (from-to)	210-217
Number of pages	8
Journal	Pulmonary Pharmacology and Therapeutics
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.pupt.2005.05.006
Publication status	Published - Jun 2006

Keywords

Adhesion molecule expression
Airway epithelial cells
Asthma
Chemokines
Ciclesonide
Cytokines

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Pharmacology

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10.1016/j.pupt.2005.05.006

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title = "Cytokine-activated bronchial epithelial cell pro-inflammatory functions are effectively downregulated in vitro by ciclesonide",

abstract = "Ciclesonide, a new inhaled corticosteroid, is administered as a parent compound and converted in the airway mucosa into the active metabolite, desisobutyryl-(des-)ciclesonide. A study was designed to evaluate the ability of ciclesonide to modulate pro-inflammatory functions of human bronchial epithelial cell (HBEC) primary cultures being converted into des-ciclesonide. HBECs were stimulated with interleukin (IL)-4 and tumour necrosis factor (TNF)-α (20 ng/mL) in the presence of ciclesonide and intercellular adhesion molecule (ICAM)-1 expression, granulocyte-macrophage colony stimulating factor (GM-CSF) and IL-8 release evaluated respectively by FACS and ELISA. Ciclesonide (3 μM) significantly inhibited ICAM-1 expression by stimulated HBECs, already after 3 h and still after 48 h culture (p",

keywords = "Adhesion molecule expression, Airway epithelial cells, Asthma, Chemokines, Ciclesonide, Cytokines",

author = "M. Silvestri and L. Serpero and L. Petecchia and F. Sabatini and F. Cerasoli and Rossi, {G. A.}",

year = "2006",

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AU - Silvestri, M.

AU - Serpero, L.

AU - Petecchia, L.

AU - Sabatini, F.

AU - Cerasoli, F.

AU - Rossi, G. A.

PY - 2006/6

Y1 - 2006/6

N2 - Ciclesonide, a new inhaled corticosteroid, is administered as a parent compound and converted in the airway mucosa into the active metabolite, desisobutyryl-(des-)ciclesonide. A study was designed to evaluate the ability of ciclesonide to modulate pro-inflammatory functions of human bronchial epithelial cell (HBEC) primary cultures being converted into des-ciclesonide. HBECs were stimulated with interleukin (IL)-4 and tumour necrosis factor (TNF)-α (20 ng/mL) in the presence of ciclesonide and intercellular adhesion molecule (ICAM)-1 expression, granulocyte-macrophage colony stimulating factor (GM-CSF) and IL-8 release evaluated respectively by FACS and ELISA. Ciclesonide (3 μM) significantly inhibited ICAM-1 expression by stimulated HBECs, already after 3 h and still after 48 h culture (p

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KW - Adhesion molecule expression

KW - Airway epithelial cells

KW - Asthma

KW - Chemokines

KW - Ciclesonide

KW - Cytokines

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ER -

Cytokine-activated bronchial epithelial cell pro-inflammatory functions are effectively downregulated in vitro by ciclesonide

Abstract

Keywords

ASJC Scopus subject areas

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