Cytokine effect on ex vivo expansion of haemopoietic stem cells from different human sources

Sandro Eridani, Umberto Mazza, Paolo Massaro, Maria Luisa La Targia, Anna Teresa Maiolo, Andrea Mosca

Research output: Contribution to journalArticle

Abstract

Human pluripotential stem cells (PSC) are currently the target for transplantation attempts and genetic manipulation. We have therefore investigated the frequency and the expansion potential of PSC's in different types of blood samples. CD 34+ cells were thus obtained from human bone marrow (BM), as well as from peripheral blood (PB) and cord blood (CB) samples. After immuno-magnetic separation the highest yields of CD 34+ cells were from BM (1.08-2.25%) and CB (0.42-1.32%) while PB samples gave much lower values. Suspension cultures of PSC's from the three sources were then set up, in the presence of combinations of haemopoietic growth factors. A remarkable amplification of the nucleated cell pool was observed reaching a maximum between 10 and 15 days of culture; earliest and maximum expansion (up to 220-fold) was achieved when Erythropoietin (Epo) was added to the culture medium, but this resulted in reduction of colony-forming cells and differentiation into erythroid progenitors. Clonogenic tests for BFU-E's derived colonies showed a peak value at 5 days of liquid culture: Further studies are advisable to establish the best cytokine combination for a valuable ex vivo expansion, coupled with preservation of stem cell properties.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalBiotherapy
Volume11
Issue number4
DOIs
Publication statusPublished - 1998

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Keywords

  • Bone marrow
  • Cord blood
  • Ex vivo expansion
  • Haemopoietic stem cells
  • Peripheral blood

ASJC Scopus subject areas

  • Pharmacology

Cite this

Eridani, S., Mazza, U., Massaro, P., Targia, M. L. L., Maiolo, A. T., & Mosca, A. (1998). Cytokine effect on ex vivo expansion of haemopoietic stem cells from different human sources. Biotherapy, 11(4), 291-296. https://doi.org/10.1023/A:1008081708054