Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen

Elisabetta Cenni, Donatella Granchi, Gabriela Ciapetti, Lucia Savarino, Alessandra Corradini, Alessandro Di Leo

Research output: Contribution to journalArticle

Abstract

In order to evaluate whether or not polyethylene terephthalate coated with pyrolytic carbon and collagen (PET+PC) favors inflammatory or hyperplastic reactions, the expression of mRNAs specific for interleukin-6 (IL-6), platelet-derived growth factor-A (PDGF-A), PDGF-B, transforming growth factor-β1 (TGF-β1), and TGF-β2 were tested in vitro by cultured human umbilical vein endothelial cells (HUVEC). The cultures were put in contact with PET+PC for 1, 24, 48, and 72 h. The same cells cultured on polystyrene without biomaterials were tested as negative controls; cultures incubated with LPS were the positive control. The expression of mRNAs was evaluated by RT-PCR with specific primers. PET+PC did not determine any differences in the expression of IL-6-specific mRNA at any of the incubation times compared to the negative control while LPS (the positive control) induced expression after 24, 48, and 72 h. PET+PC induced a more precocious expression of mRNA specific for PDGF-A than did the negative control; however, the expression no longer was present after 48 h while in the negative control the expression stopped after 72 h. PET+PC induced a less frequent expression of PDGF-B-specific mRNA than did the negative control and LPS, especially after 24 h. PET+PC induced a later expression of TGF-β2- specific mRNA than did the negative control and a less frequent expression of mRNA specific for TGF-β1 after 24 and 72 h. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish
Pages (from-to)483-489
Number of pages7
JournalJournal of Biomedical Materials Research
Volume50
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

Polyethylene Terephthalates
Endothelial cells
Collagen
Polyethylene terephthalates
Endothelial Cells
Cytokines
Messenger RNA
Carbon
Transforming Growth Factors
Platelets
Interleukin-6
Polystyrenes
Human Umbilical Vein Endothelial Cells
Biocompatible Materials
In Vitro Techniques
pyrolytic carbon
Cell culture
Cultured Cells
Biomaterials
Polymerase Chain Reaction

Keywords

  • Endothelial cell
  • IL-6
  • PDGF
  • Pyrolytic carbon
  • TGF-β

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen. / Cenni, Elisabetta; Granchi, Donatella; Ciapetti, Gabriela; Savarino, Lucia; Corradini, Alessandra; Di Leo, Alessandro.

In: Journal of Biomedical Materials Research, Vol. 50, No. 4, 2000, p. 483-489.

Research output: Contribution to journalArticle

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