Cytokine gene expression and T-cell proliferative responses in lymph node mononuclear cells from children with early stage human immunodeficiency virus infection

I. Airoldi, D. Saverino, A. Favre, F. Ghiotto, C. Tacchetti, P. Facchetti, G. Piatti, G. Li Pira, D. Fenoglio, M. Duse, E. Ciccone, F. Manca, A. Plebani, C. E. Grossi, V. Pistoia

Research output: Contribution to journalArticle

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Abstract

Background and Objectives. The immunologic events taking place in secondary lymphoid tissue from children with early stage human immunodeficiency virus (HIV) infection are poorly understood. The aim of this study was to investigate cytokine gene expression and proliferative responses in lymph node (LN) biopsies from five children with early stage HIV infection, in the context of LN morphology and viral load. Design and Methods. The design of the study was approved by the local Ethical Committee. Cytokine gene expression was studied in LN biopsies and in paired peripheral blood (PB) samples from HIV-infected children by reverse transcriptase-polymerase chain reaction. T-cell proliferation was assessed by 3H-thymidine incorporation. Viral burden in germinal centers was assessed by video densitometric analysis following immunohistochemical staining for HIV p24. Results. Interleukin (IL)-2, IL-4 and IL-5 mRNA were not detected in any LN or PB sample from HIV-infected children. Interferon (IFN)-γ mRNA was found only in CD8+ cells. IL-12 p35, IL-10, transforming growth factor-(TGF)-β1, regulated on activation normal T-cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1α, MIP-1β and IL-16 transcripts were detected in all samples. Proliferation of LN and PB mononuclear cells to polyclonal mitogens and soluble (recall and HIV-related) antigens was impaired as compared with the responses in a group of age-matched healthy controls. Interpretation and Conclusions. Changes in cytokine gene expression and T-cell proliferative responses are already detectable in lymph nodes from HIV-infected children at an early stage of disease.

Original languageEnglish
Pages (from-to)1237-1247
Number of pages11
JournalHaematologica
Volume85
Issue number12
Publication statusPublished - 2000

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Virus Diseases
Lymph Nodes
HIV
Cytokines
T-Lymphocytes
Gene Expression
Macrophage Inflammatory Proteins
Viral Load
Interleukin-12 Subunit p35
Interleukin-16
Biopsy
Messenger RNA
Germinal Center
Interleukin-5
Transforming Growth Factors
Lymphoid Tissue
Reverse Transcriptase Polymerase Chain Reaction
Mitogens
Interleukin-4
Interleukin-10

Keywords

  • Lymph nodes
  • Pediatric HIV infection

ASJC Scopus subject areas

  • Hematology

Cite this

Cytokine gene expression and T-cell proliferative responses in lymph node mononuclear cells from children with early stage human immunodeficiency virus infection. / Airoldi, I.; Saverino, D.; Favre, A.; Ghiotto, F.; Tacchetti, C.; Facchetti, P.; Piatti, G.; Li Pira, G.; Fenoglio, D.; Duse, M.; Ciccone, E.; Manca, F.; Plebani, A.; Grossi, C. E.; Pistoia, V.

In: Haematologica, Vol. 85, No. 12, 2000, p. 1237-1247.

Research output: Contribution to journalArticle

Airoldi, I, Saverino, D, Favre, A, Ghiotto, F, Tacchetti, C, Facchetti, P, Piatti, G, Li Pira, G, Fenoglio, D, Duse, M, Ciccone, E, Manca, F, Plebani, A, Grossi, CE & Pistoia, V 2000, 'Cytokine gene expression and T-cell proliferative responses in lymph node mononuclear cells from children with early stage human immunodeficiency virus infection', Haematologica, vol. 85, no. 12, pp. 1237-1247.
Airoldi, I. ; Saverino, D. ; Favre, A. ; Ghiotto, F. ; Tacchetti, C. ; Facchetti, P. ; Piatti, G. ; Li Pira, G. ; Fenoglio, D. ; Duse, M. ; Ciccone, E. ; Manca, F. ; Plebani, A. ; Grossi, C. E. ; Pistoia, V. / Cytokine gene expression and T-cell proliferative responses in lymph node mononuclear cells from children with early stage human immunodeficiency virus infection. In: Haematologica. 2000 ; Vol. 85, No. 12. pp. 1237-1247.
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T1 - Cytokine gene expression and T-cell proliferative responses in lymph node mononuclear cells from children with early stage human immunodeficiency virus infection

AU - Airoldi, I.

AU - Saverino, D.

AU - Favre, A.

AU - Ghiotto, F.

AU - Tacchetti, C.

AU - Facchetti, P.

AU - Piatti, G.

AU - Li Pira, G.

AU - Fenoglio, D.

AU - Duse, M.

AU - Ciccone, E.

AU - Manca, F.

AU - Plebani, A.

AU - Grossi, C. E.

AU - Pistoia, V.

PY - 2000

Y1 - 2000

N2 - Background and Objectives. The immunologic events taking place in secondary lymphoid tissue from children with early stage human immunodeficiency virus (HIV) infection are poorly understood. The aim of this study was to investigate cytokine gene expression and proliferative responses in lymph node (LN) biopsies from five children with early stage HIV infection, in the context of LN morphology and viral load. Design and Methods. The design of the study was approved by the local Ethical Committee. Cytokine gene expression was studied in LN biopsies and in paired peripheral blood (PB) samples from HIV-infected children by reverse transcriptase-polymerase chain reaction. T-cell proliferation was assessed by 3H-thymidine incorporation. Viral burden in germinal centers was assessed by video densitometric analysis following immunohistochemical staining for HIV p24. Results. Interleukin (IL)-2, IL-4 and IL-5 mRNA were not detected in any LN or PB sample from HIV-infected children. Interferon (IFN)-γ mRNA was found only in CD8+ cells. IL-12 p35, IL-10, transforming growth factor-(TGF)-β1, regulated on activation normal T-cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1α, MIP-1β and IL-16 transcripts were detected in all samples. Proliferation of LN and PB mononuclear cells to polyclonal mitogens and soluble (recall and HIV-related) antigens was impaired as compared with the responses in a group of age-matched healthy controls. Interpretation and Conclusions. Changes in cytokine gene expression and T-cell proliferative responses are already detectable in lymph nodes from HIV-infected children at an early stage of disease.

AB - Background and Objectives. The immunologic events taking place in secondary lymphoid tissue from children with early stage human immunodeficiency virus (HIV) infection are poorly understood. The aim of this study was to investigate cytokine gene expression and proliferative responses in lymph node (LN) biopsies from five children with early stage HIV infection, in the context of LN morphology and viral load. Design and Methods. The design of the study was approved by the local Ethical Committee. Cytokine gene expression was studied in LN biopsies and in paired peripheral blood (PB) samples from HIV-infected children by reverse transcriptase-polymerase chain reaction. T-cell proliferation was assessed by 3H-thymidine incorporation. Viral burden in germinal centers was assessed by video densitometric analysis following immunohistochemical staining for HIV p24. Results. Interleukin (IL)-2, IL-4 and IL-5 mRNA were not detected in any LN or PB sample from HIV-infected children. Interferon (IFN)-γ mRNA was found only in CD8+ cells. IL-12 p35, IL-10, transforming growth factor-(TGF)-β1, regulated on activation normal T-cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1α, MIP-1β and IL-16 transcripts were detected in all samples. Proliferation of LN and PB mononuclear cells to polyclonal mitogens and soluble (recall and HIV-related) antigens was impaired as compared with the responses in a group of age-matched healthy controls. Interpretation and Conclusions. Changes in cytokine gene expression and T-cell proliferative responses are already detectable in lymph nodes from HIV-infected children at an early stage of disease.

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KW - Pediatric HIV infection

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