Abstract
Cytokine gene transfer into mouse tumor cells has been shown to stimulate a strong immune response resulting in the rejection of the transduced tumor when injected in vivo. Therefore, retroviral vectors containing the human interleukin (IL)-2 or IL-4 gene have been constructed to transduce human melanoma cells to explore whether their immunogenicity can be increased both in vitro and in vivo. Our preliminary results indicate that retroviral vectors can efficiently transduce the IL-2 or IL-4 gene into melanoma clones, inducing production of either cytokine in the range of 0.5-2 ng/ml/105 cells in 48-72 h. No modifications of the growth rate, morphology and antigenicity of the transduced tumor cells were found.
| Original language | English |
|---|---|
| Pages (from-to) | 76-84 |
| Number of pages | 9 |
| Journal | Natural Immunity |
| Volume | 13 |
| Issue number | 2-3 |
| Publication status | Published - 1994 |
Fingerprint
Keywords
- Cytokines
- Gene transduction
- Immunotherapy of melanoma
- Retroviral vectors
ASJC Scopus subject areas
- Immunology
Cite this
Cytokine gene transduction in tumor cells : Interleukin (IL)-2 or IL-4 gene transfer in human melanoma cells. / Melani, C.; Chiodoni, C.; Arienti, F.; Maccalli, C.; Sule-Suso, J.; Anichini, A.; Colombo, M. P.; Parmiani, G.
In: Natural Immunity, Vol. 13, No. 2-3, 1994, p. 76-84.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytokine gene transduction in tumor cells
T2 - Interleukin (IL)-2 or IL-4 gene transfer in human melanoma cells
AU - Melani, C.
AU - Chiodoni, C.
AU - Arienti, F.
AU - Maccalli, C.
AU - Sule-Suso, J.
AU - Anichini, A.
AU - Colombo, M. P.
AU - Parmiani, G.
PY - 1994
Y1 - 1994
N2 - Cytokine gene transfer into mouse tumor cells has been shown to stimulate a strong immune response resulting in the rejection of the transduced tumor when injected in vivo. Therefore, retroviral vectors containing the human interleukin (IL)-2 or IL-4 gene have been constructed to transduce human melanoma cells to explore whether their immunogenicity can be increased both in vitro and in vivo. Our preliminary results indicate that retroviral vectors can efficiently transduce the IL-2 or IL-4 gene into melanoma clones, inducing production of either cytokine in the range of 0.5-2 ng/ml/105 cells in 48-72 h. No modifications of the growth rate, morphology and antigenicity of the transduced tumor cells were found.
AB - Cytokine gene transfer into mouse tumor cells has been shown to stimulate a strong immune response resulting in the rejection of the transduced tumor when injected in vivo. Therefore, retroviral vectors containing the human interleukin (IL)-2 or IL-4 gene have been constructed to transduce human melanoma cells to explore whether their immunogenicity can be increased both in vitro and in vivo. Our preliminary results indicate that retroviral vectors can efficiently transduce the IL-2 or IL-4 gene into melanoma clones, inducing production of either cytokine in the range of 0.5-2 ng/ml/105 cells in 48-72 h. No modifications of the growth rate, morphology and antigenicity of the transduced tumor cells were found.
KW - Cytokines
KW - Gene transduction
KW - Immunotherapy of melanoma
KW - Retroviral vectors
UR - http://www.scopus.com/inward/record.url?scp=0028261684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028261684&partnerID=8YFLogxK
M3 - Article
C2 - 8173238
AN - SCOPUS:0028261684
VL - 13
SP - 76
EP - 84
JO - Natural Immunity
JF - Natural Immunity
SN - 1018-8916
IS - 2-3
ER -