Cytokine gene transduction in tumor cells: Interleukin (IL)-2 or IL-4 gene transfer in human melanoma cells

C. Melani, C. Chiodoni, F. Arienti, C. Maccalli, J. Sule-Suso, A. Anichini, M. P. Colombo, G. Parmiani

Research output: Contribution to journalArticle

Abstract

Cytokine gene transfer into mouse tumor cells has been shown to stimulate a strong immune response resulting in the rejection of the transduced tumor when injected in vivo. Therefore, retroviral vectors containing the human interleukin (IL)-2 or IL-4 gene have been constructed to transduce human melanoma cells to explore whether their immunogenicity can be increased both in vitro and in vivo. Our preliminary results indicate that retroviral vectors can efficiently transduce the IL-2 or IL-4 gene into melanoma clones, inducing production of either cytokine in the range of 0.5-2 ng/ml/105 cells in 48-72 h. No modifications of the growth rate, morphology and antigenicity of the transduced tumor cells were found.

Original languageEnglish
Pages (from-to)76-84
Number of pages9
JournalNatural Immunity
Volume13
Issue number2-3
Publication statusPublished - 1994

Keywords

  • Cytokines
  • Gene transduction
  • Immunotherapy of melanoma
  • Retroviral vectors

ASJC Scopus subject areas

  • Immunology

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