Abstract
Selective IgA deficiency (IgAD) and celiac disease (CD) are frequently associated and share the ancestral haplotype human leukocyte antigen (HLA)-8.1, which is characterized by a peculiar cytokine profile. The aim of this study was to evaluate the role of tumor necrosis factor (TNF) and interleukin (IL)-10 alleles in CD and CD-IgAD. METHODS: The distribution of some biallelic polymorphisms of both cytokine promoters (-308G→A and -863C→-A at TNF promoter sequence and -1082G→A, -819C→A, and -592C→T at IL-10 promoter) were typed using biotilinated specific probes in 32 celiac patients, in 34 CD-IgAD patients, and in 96 healthy controls. RESULTS: In CD and CD-IgAD, the -308A allele was significantly more frequent than in controls, whereas no significant differences were observed for the biallelic polymorphisms at the -863 and for the three IL-10 promoter polymorphisms. The evaluation of combined TNF and IL-10 genotypes showed in CD-IgAD a significant reduction of -308G/- 1082G homozygous subjects and both in CD and CD-IgAD groups an increase of 308AA/1082GG. Accordingly, CD-IgAD patients positive both for -308A TNF and - 1082A IL-10 showed an increase of TNF-α and a reduction of IL-10 serum levels. CONCLUSIONS: Genetically determined increased production of TNF-α and reduction of IL-10 may be relevant for susceptibility to CD, mainly in IgAD, as the different allele expression at TNF and IL-10 loci seems to influence cytokine production profile.
| Original language | English |
|---|---|
| Pages (from-to) | 850-856 |
| Number of pages | 7 |
| Journal | American Journal of Gastroenterology |
| Volume | 98 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2003 |
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ASJC Scopus subject areas
- Gastroenterology
Cite this
Cytokine genotyping (TNF and IL-10) in patients with celiac disease and selective IgA deficiency. / Cataldo, F.; Lio, D.; Marino, V.; Scola, L.; Crivello, A.; Mulè, A. M.; Corazza, G. R.; Catassi, C.; Fabiani, E.; Baldassarre, M.; Corvaglia, L.; Lazzari, R.; Spina, M.; Guandalini, S.; Pensabene, L.; Brusa, S.; Gentile, T.; Barera, G.; Bianchi, C.; Rea, F.; Di Stefano, P.; Trippiedi, M. A.; Greco, P.; Di Sabatino, A.; Pelli, A. M.; Barbato, M.; Bonamico, M.; Viola, F.; Ambruzzi, A. M.; D'Altilia, M. R.; Musumeci, S.; Ansaldi, N.; Bramante, L.; Sategna-Guidetti, C.; Nassimbeni, G.; Ventura, A.
In: American Journal of Gastroenterology, Vol. 98, No. 4, 01.04.2003, p. 850-856.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Cytokine genotyping (TNF and IL-10) in patients with celiac disease and selective IgA deficiency
AU - Cataldo, F.
AU - Lio, D.
AU - Marino, V.
AU - Scola, L.
AU - Crivello, A.
AU - Mulè, A. M.
AU - Corazza, G. R.
AU - Catassi, C.
AU - Fabiani, E.
AU - Baldassarre, M.
AU - Corvaglia, L.
AU - Lazzari, R.
AU - Spina, M.
AU - Guandalini, S.
AU - Pensabene, L.
AU - Brusa, S.
AU - Gentile, T.
AU - Barera, G.
AU - Bianchi, C.
AU - Rea, F.
AU - Di Stefano, P.
AU - Trippiedi, M. A.
AU - Greco, P.
AU - Di Sabatino, A.
AU - Pelli, A. M.
AU - Barbato, M.
AU - Bonamico, M.
AU - Viola, F.
AU - Ambruzzi, A. M.
AU - D'Altilia, M. R.
AU - Musumeci, S.
AU - Ansaldi, N.
AU - Bramante, L.
AU - Sategna-Guidetti, C.
AU - Nassimbeni, G.
AU - Ventura, A.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Selective IgA deficiency (IgAD) and celiac disease (CD) are frequently associated and share the ancestral haplotype human leukocyte antigen (HLA)-8.1, which is characterized by a peculiar cytokine profile. The aim of this study was to evaluate the role of tumor necrosis factor (TNF) and interleukin (IL)-10 alleles in CD and CD-IgAD. METHODS: The distribution of some biallelic polymorphisms of both cytokine promoters (-308G→A and -863C→-A at TNF promoter sequence and -1082G→A, -819C→A, and -592C→T at IL-10 promoter) were typed using biotilinated specific probes in 32 celiac patients, in 34 CD-IgAD patients, and in 96 healthy controls. RESULTS: In CD and CD-IgAD, the -308A allele was significantly more frequent than in controls, whereas no significant differences were observed for the biallelic polymorphisms at the -863 and for the three IL-10 promoter polymorphisms. The evaluation of combined TNF and IL-10 genotypes showed in CD-IgAD a significant reduction of -308G/- 1082G homozygous subjects and both in CD and CD-IgAD groups an increase of 308AA/1082GG. Accordingly, CD-IgAD patients positive both for -308A TNF and - 1082A IL-10 showed an increase of TNF-α and a reduction of IL-10 serum levels. CONCLUSIONS: Genetically determined increased production of TNF-α and reduction of IL-10 may be relevant for susceptibility to CD, mainly in IgAD, as the different allele expression at TNF and IL-10 loci seems to influence cytokine production profile.
AB - Selective IgA deficiency (IgAD) and celiac disease (CD) are frequently associated and share the ancestral haplotype human leukocyte antigen (HLA)-8.1, which is characterized by a peculiar cytokine profile. The aim of this study was to evaluate the role of tumor necrosis factor (TNF) and interleukin (IL)-10 alleles in CD and CD-IgAD. METHODS: The distribution of some biallelic polymorphisms of both cytokine promoters (-308G→A and -863C→-A at TNF promoter sequence and -1082G→A, -819C→A, and -592C→T at IL-10 promoter) were typed using biotilinated specific probes in 32 celiac patients, in 34 CD-IgAD patients, and in 96 healthy controls. RESULTS: In CD and CD-IgAD, the -308A allele was significantly more frequent than in controls, whereas no significant differences were observed for the biallelic polymorphisms at the -863 and for the three IL-10 promoter polymorphisms. The evaluation of combined TNF and IL-10 genotypes showed in CD-IgAD a significant reduction of -308G/- 1082G homozygous subjects and both in CD and CD-IgAD groups an increase of 308AA/1082GG. Accordingly, CD-IgAD patients positive both for -308A TNF and - 1082A IL-10 showed an increase of TNF-α and a reduction of IL-10 serum levels. CONCLUSIONS: Genetically determined increased production of TNF-α and reduction of IL-10 may be relevant for susceptibility to CD, mainly in IgAD, as the different allele expression at TNF and IL-10 loci seems to influence cytokine production profile.
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UR - http://www.scopus.com/inward/citedby.url?scp=0037598800&partnerID=8YFLogxK
U2 - 10.1111/j.1572-0241.2003.t01-1-07377.x
DO - 10.1111/j.1572-0241.2003.t01-1-07377.x
M3 - Article
C2 - 12738467
AN - SCOPUS:0037598800
VL - 98
SP - 850
EP - 856
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
SN - 0002-9270
IS - 4
ER -