Cytokine Loaded Biopolymers as a Novel Strategy to Study Stem Cells during Wound-Healing Processes

Annalisa Grimaldi, Serena Banfi, Jacopo Vizioli, Gianluca Tettamanti, Douglas M. Noonan, Magda de Eguileor

Research output: Contribution to journalArticle

Abstract

The biopolymer matrigel loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. Data demonstrate that the injection of the matrigel biopolymer supplemented with interleukin-8 (IL-8) in the leech Hirudo medicinalis can be used to purify cell populations showing the same morphofunctional and molecular mechanisms of specific populations of vertebrate hematopoietic precursor cells involved in tissue repair. These cells spontaneously differentiated into myofibroblasts. This approach highlights how the innovative use of a cytokine-loaded biopolymer for an in vivo cell sorting method, applied to a simple invertebrate model, can be a tool for studying myofibroblast cell biology and its regulation, step by step. The biopolymer matrigel loaded with a specific cytokine is used for the recruitment in vivo of specific cell populations and as a vector to prepare cell tissue cultures. The injection of the matrigel biopolymer supplemented with interleukin-8 (IL-8) in the leech Hirudo medicinalis is a remarkably effective tool for isolating a specific population of precursor cells, which in turn can differentiate into myofibroblastlike cells.

Original languageEnglish
Pages (from-to)1008-1019
Number of pages12
JournalMacromolecular Bioscience
Volume11
Issue number8
DOIs
Publication statusPublished - Aug 11 2011

Keywords

  • Biopolymers
  • Interleukin-8
  • Leech
  • Myofibroblasts
  • Wound healing

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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