Cytokine production and adhesive protein expression by endothelial cells after contact with polyethylene terephthalate

E. Cenni; D. Granchi; G. Ciapetti; E. Verri; D. Cavedagna; S. Gamberini; A. Di Leo; A. Pizzoferrato

doi:10.1016/0142-9612(96)00037-3

Cytokine production and adhesive protein expression by endothelial cells after contact with polyethylene terephthalate

E. Cenni, D. Granchi, G. Ciapetti, E. Verri, D. Cavedagna, S. Gamberini, A. Di Leo, A. Pizzoferrato

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article

Abstract

The authors have evaluated adhesive protein expression and cytokine production by human umbilical vein endothelial cells cultured in contact with polyethylene terephthalate (PET). ELAM-1, ICAM-1 and VCAM-1 expression was determined by flow cytometry; the concentration of interleukin-1α (IL-1α), interleukin-6 (IL-6), granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF) in the supernatant was determined by enzyme immunoassay. The contact with PET determined a significant increase in ELAM-1 expression and insignificant increase in cytokine production, demonstrating that PET had a limited capability to stimulate endothelial cells in a pro-inflammatory sense.

Original language	English
Pages (from-to)	2071-2076
Number of pages	6
Journal	Biomaterials
Volume	17
Issue number	21
DOIs	https://doi.org/10.1016/0142-9612(96)00037-3
Publication status	Published - Nov 1996

Keywords

Adhesive protein
Cytokine
Endothelial cells
Polyethylene terephthalate

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

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Cenni, E., Granchi, D., Ciapetti, G., Verri, E., Cavedagna, D., Gamberini, S., Di Leo, A., & Pizzoferrato, A. (1996). Cytokine production and adhesive protein expression by endothelial cells after contact with polyethylene terephthalate. Biomaterials, 17(21), 2071-2076. https://doi.org/10.1016/0142-9612(96)00037-3

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abstract = "The authors have evaluated adhesive protein expression and cytokine production by human umbilical vein endothelial cells cultured in contact with polyethylene terephthalate (PET). ELAM-1, ICAM-1 and VCAM-1 expression was determined by flow cytometry; the concentration of interleukin-1α (IL-1α), interleukin-6 (IL-6), granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF) in the supernatant was determined by enzyme immunoassay. The contact with PET determined a significant increase in ELAM-1 expression and insignificant increase in cytokine production, demonstrating that PET had a limited capability to stimulate endothelial cells in a pro-inflammatory sense.",

keywords = "Adhesive protein, Cytokine, Endothelial cells, Polyethylene terephthalate",

author = "E. Cenni and D. Granchi and G. Ciapetti and E. Verri and D. Cavedagna and S. Gamberini and {Di Leo}, A. and A. Pizzoferrato",

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AU - Cenni, E.

AU - Granchi, D.

AU - Ciapetti, G.

AU - Verri, E.

AU - Cavedagna, D.

AU - Gamberini, S.

AU - Di Leo, A.

AU - Pizzoferrato, A.

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AB - The authors have evaluated adhesive protein expression and cytokine production by human umbilical vein endothelial cells cultured in contact with polyethylene terephthalate (PET). ELAM-1, ICAM-1 and VCAM-1 expression was determined by flow cytometry; the concentration of interleukin-1α (IL-1α), interleukin-6 (IL-6), granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF) in the supernatant was determined by enzyme immunoassay. The contact with PET determined a significant increase in ELAM-1 expression and insignificant increase in cytokine production, demonstrating that PET had a limited capability to stimulate endothelial cells in a pro-inflammatory sense.

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KW - Cytokine

KW - Endothelial cells

KW - Polyethylene terephthalate

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