Cytokine production from peripheral blood mononuclear cells and polymorphonuclear leukocytes in patients studied for suspected obstructive sleep apnea

Luigina Guasti, Franca Marino, Marco Cosentino, Lorenzo Maroni, Andrea M. Maresca, Fausto Colombo, Ramona C. Maio, Luana Castiglioni, Federica Saporiti, Anna Loraschi, Giovanni Gaudio, Antonella Bernasconi, Emanuela Laurita, Anna M. Grandi, Achille Venco

Research output: Contribution to journalArticle

Abstract

Purpose: The relationship between obstructive sleep apnea (OSA) and atherosclerosis-related inflammation has been poorly investigated, particularly focusing on functional responses of immune cells playing a key role in atherogenesis and in comparison with control groups with similar cardiovascular risk factors which are known to be themselves associated with inflammation. We sought to determine cellular tumor necrosis factor-alpha (TNF-α) production from peripheral blood mononuclear cells (PBMCs) and interleukin (IL)-8 release from neutrophils (PMNs) in patients studied for suspected OSA. Methods: Thirty-six consecutive patients who underwent a nocturnal complete cardiorespiratory evaluation for suspected OSA were initially evaluated. Serum, PBMCs, and PMNs were isolated (at baseline and after 12 weeks) from patients with apnea-ipopnea index (AHI) >20 (OSA group, n=16) and from control patients with AHI

Original languageEnglish
Pages (from-to)3-11
Number of pages9
JournalSleep and Breathing
Volume15
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Continuous positive airway pressure ventilation
  • Interleukin-8
  • Obstructive sleep apnea
  • Peripheral blood mononuclear cells
  • Polymorphonuclear leukocytes
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Clinical Neurology

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  • Cite this

    Guasti, L., Marino, F., Cosentino, M., Maroni, L., Maresca, A. M., Colombo, F., Maio, R. C., Castiglioni, L., Saporiti, F., Loraschi, A., Gaudio, G., Bernasconi, A., Laurita, E., Grandi, A. M., & Venco, A. (2011). Cytokine production from peripheral blood mononuclear cells and polymorphonuclear leukocytes in patients studied for suspected obstructive sleep apnea. Sleep and Breathing, 15(1), 3-11. https://doi.org/10.1007/s11325-009-0315-x