Cytokine production (IL-1α, IL-1β, and TNFα) and endothelial cell activation (ELAM-1 and HLA-DR) in reactive lymphadenitis, Hodgkin's Disease, and in non-Hodgkin's lymphomas. An immunocytochemical study

L. P. Ruco, D. Pomponi, R. Pigott, A. Stoppacciaro, F. Monardo, S. Uccini, D. Boraschi, A. Tagliabue, A. Santoni, E. Dejana, A. Mantovani, C. D. Baroni

Research output: Contribution to journalArticle

Abstract

Cryostat sections of 58 lymph nodes were immunostained with a polyclonal rabbit serum against Il-1α, and with monoclonal antibodies directed to IL-1α (Vmp18), IL-1β (Vhp20 and BrhC3), and tumor necrosis factor α (TNFα) (B154.7). Furthermore the presence of cytokine-containing cells was correlated with the expression of endothelial leukocyte adhesion molecule (ELAM-1; 29F2) and of human leukocyte antigen (HLA-DR) (OKIa-1) by endothelial cells. Cells containing IL-1 and/or TNFα were detected mainly in pathologic conditions characterized by reactive or neoplastic expansion of the lymph node paracortex. Cells positive for IL-1 were detected in 16 of 21 cases of Hodgkin's disease, in 4 of 4 cases of T-NHL, and in 5 cases of diffuse or mixed lymphadenitis. Interleukin-1α was detected in macrophages, interdigitating reticulum cells (IDRCs), endothelial cells, and neoplastic Hodgkin's and Reed-Sternberg (H-RS) cells. Cells positive for IL-1β were much fewer and consisted mainly of macrophages. Hodgkin's Reed-Sternberg cells were negative for IL-1β even after in vitro stimulation with bacterial endotoxin. Tumor necrosis factor α (TNFα) was present in macrophages and H-RS cells. Endothelial leukocyte adhesion molecule-1 expression by endothelial venules was detected in 17 of 20 cases of Hodgkin's disease, in 2 of 4 cases of T-NHL, and in 5 of 5 cases of diffuse lymphadenitis. In these pathologic conditions, HLA-DR antigens also were expressed frequently by endothelial cells. Cytokine-containing cells and ELAM-1-positive high endothelial venules (HEV) were extremely rare in lymph nodes involved by follicular lymphadenitis (12 cases) or B-NHL (16 cases). In cases of reactive or neoplastic B-cell proliferations, HLA-DR-positive HEVs still were present often. Our results indicate that IL-1/TNFα production at tissue level is often associated with ELAM-1 expression by HEVs, but is less well correlated with expression of HLA-DR antigens by endothelial cells.

Original languageEnglish
Pages (from-to)1163-1171
Number of pages9
JournalAmerican Journal of Pathology
Volume137
Issue number5
Publication statusPublished - 1990

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Lymphadenitis
E-Selectin
HLA-DR Antigens
Hodgkin Disease
Interleukin-1
Non-Hodgkin's Lymphoma
Endothelial Cells
Tumor Necrosis Factor-alpha
Cytokines
Reed-Sternberg Cells
Venules
Lymph Nodes
Macrophages
Reticulum
HLA Antigens
Endotoxins
Dendritic Cells
B-Lymphocytes
Monoclonal Antibodies
Cell Proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cytokine production (IL-1α, IL-1β, and TNFα) and endothelial cell activation (ELAM-1 and HLA-DR) in reactive lymphadenitis, Hodgkin's Disease, and in non-Hodgkin's lymphomas. An immunocytochemical study. / Ruco, L. P.; Pomponi, D.; Pigott, R.; Stoppacciaro, A.; Monardo, F.; Uccini, S.; Boraschi, D.; Tagliabue, A.; Santoni, A.; Dejana, E.; Mantovani, A.; Baroni, C. D.

In: American Journal of Pathology, Vol. 137, No. 5, 1990, p. 1163-1171.

Research output: Contribution to journalArticle

Ruco, LP, Pomponi, D, Pigott, R, Stoppacciaro, A, Monardo, F, Uccini, S, Boraschi, D, Tagliabue, A, Santoni, A, Dejana, E, Mantovani, A & Baroni, CD 1990, 'Cytokine production (IL-1α, IL-1β, and TNFα) and endothelial cell activation (ELAM-1 and HLA-DR) in reactive lymphadenitis, Hodgkin's Disease, and in non-Hodgkin's lymphomas. An immunocytochemical study', American Journal of Pathology, vol. 137, no. 5, pp. 1163-1171.
Ruco, L. P. ; Pomponi, D. ; Pigott, R. ; Stoppacciaro, A. ; Monardo, F. ; Uccini, S. ; Boraschi, D. ; Tagliabue, A. ; Santoni, A. ; Dejana, E. ; Mantovani, A. ; Baroni, C. D. / Cytokine production (IL-1α, IL-1β, and TNFα) and endothelial cell activation (ELAM-1 and HLA-DR) in reactive lymphadenitis, Hodgkin's Disease, and in non-Hodgkin's lymphomas. An immunocytochemical study. In: American Journal of Pathology. 1990 ; Vol. 137, No. 5. pp. 1163-1171.
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T1 - Cytokine production (IL-1α, IL-1β, and TNFα) and endothelial cell activation (ELAM-1 and HLA-DR) in reactive lymphadenitis, Hodgkin's Disease, and in non-Hodgkin's lymphomas. An immunocytochemical study

AU - Ruco, L. P.

AU - Pomponi, D.

AU - Pigott, R.

AU - Stoppacciaro, A.

AU - Monardo, F.

AU - Uccini, S.

AU - Boraschi, D.

AU - Tagliabue, A.

AU - Santoni, A.

AU - Dejana, E.

AU - Mantovani, A.

AU - Baroni, C. D.

PY - 1990

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N2 - Cryostat sections of 58 lymph nodes were immunostained with a polyclonal rabbit serum against Il-1α, and with monoclonal antibodies directed to IL-1α (Vmp18), IL-1β (Vhp20 and BrhC3), and tumor necrosis factor α (TNFα) (B154.7). Furthermore the presence of cytokine-containing cells was correlated with the expression of endothelial leukocyte adhesion molecule (ELAM-1; 29F2) and of human leukocyte antigen (HLA-DR) (OKIa-1) by endothelial cells. Cells containing IL-1 and/or TNFα were detected mainly in pathologic conditions characterized by reactive or neoplastic expansion of the lymph node paracortex. Cells positive for IL-1 were detected in 16 of 21 cases of Hodgkin's disease, in 4 of 4 cases of T-NHL, and in 5 cases of diffuse or mixed lymphadenitis. Interleukin-1α was detected in macrophages, interdigitating reticulum cells (IDRCs), endothelial cells, and neoplastic Hodgkin's and Reed-Sternberg (H-RS) cells. Cells positive for IL-1β were much fewer and consisted mainly of macrophages. Hodgkin's Reed-Sternberg cells were negative for IL-1β even after in vitro stimulation with bacterial endotoxin. Tumor necrosis factor α (TNFα) was present in macrophages and H-RS cells. Endothelial leukocyte adhesion molecule-1 expression by endothelial venules was detected in 17 of 20 cases of Hodgkin's disease, in 2 of 4 cases of T-NHL, and in 5 of 5 cases of diffuse lymphadenitis. In these pathologic conditions, HLA-DR antigens also were expressed frequently by endothelial cells. Cytokine-containing cells and ELAM-1-positive high endothelial venules (HEV) were extremely rare in lymph nodes involved by follicular lymphadenitis (12 cases) or B-NHL (16 cases). In cases of reactive or neoplastic B-cell proliferations, HLA-DR-positive HEVs still were present often. Our results indicate that IL-1/TNFα production at tissue level is often associated with ELAM-1 expression by HEVs, but is less well correlated with expression of HLA-DR antigens by endothelial cells.

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