Cytokine release, small airway injury, and parenchymal damage during mechanical ventilation in normal open-chest rats

Edgardo D'Angelo, Antonia Koutsoukou, Patrizia Della Valle, Guendalina Gentile, Matteo Pecchiari

Research output: Contribution to journalArticle

Abstract

Lung morpho-functional alterations and inflammatory response to various types of mechanical ventilation (MV) have been assessed in normal, anesthetized, openchest rats. Measurements were taken during protective MV [tidal volume (VT) = 8 ml/kg; positive end-expiratory pressure (PEEP) = 2.6 cmH2O] before and after a 2- to 2.5-h period of ventilation on PEEP (control group), zero EEP without (ZEEP group) or with administration of dioctylsodiumsulfosuccinate (ZEEP-DOSS group), on negative EEP (NEEP group), or with large VT (26 ml/kg) on PEEP (Hi-VT group). No change in lung mechanics occurred in the Control group. Relative to the initial period of MV on PEEP, airway resistance increased by 33 ± 4, 49 ± 9, 573 ± 84, and 13 ± 4%, and quasi-static elastance by 19 ± 3, 35 ± 7, 248 ± 12, and 20 ± 3% in the ZEEP, NEEP, ZEEP-DOSS, and Hi-VT groups. Relative to Control, all groups ventilated from low lung volumes exhibited histologic signs of bronchiolar injury, more marked in the NEEP and ZEEP-DOSS groups. Parenchymal and vascular injury occurred in the ZEEP-DOSS and Hi-VT groups. Pro-inflammatory cytokine concentration in the bronchoalveolar lavage fluid (BALF) was similar in the Control and ZEEP group, but increased in all other groups, and higher in the ZEEP-DOSS and Hi-VT groups. Interrupter resistance was correlated with indexes of bronchiolar damage, and cytokine levels with vascular-alveolar damage, as indexed by lung wet-to-dry ratio. Hence, protective MV from resting lung volume causes mechanical alterations and small airway injury, but no cytokine release, which seems mainly related to stress-related damage of endothelial-alveolar cells. Enhanced small airway epithelial damage with induced surfactant dysfunction or MV on NEEP can, however, contribute to cytokine production.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalJournal of Applied Physiology
Volume104
Issue number1
DOIs
Publication statusPublished - Jan 2008

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Dioctyl Sulfosuccinic Acid
Artificial Respiration
Positive-Pressure Respiration
Thorax
Cytokines
Wounds and Injuries
Lung
Control Groups
Alveolar Epithelial Cells
Airway Resistance
Vascular System Injuries
Tidal Volume
Bronchoalveolar Lavage Fluid
Pulmonary Edema
Mechanics
Blood Vessels
Ventilation
Endothelial Cells

Keywords

  • Inflammation
  • Lung mechanics
  • Microvascular damage
  • Recruitment-derecruitment of lung units

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Cytokine release, small airway injury, and parenchymal damage during mechanical ventilation in normal open-chest rats. / D'Angelo, Edgardo; Koutsoukou, Antonia; Della Valle, Patrizia; Gentile, Guendalina; Pecchiari, Matteo.

In: Journal of Applied Physiology, Vol. 104, No. 1, 01.2008, p. 41-49.

Research output: Contribution to journalArticle

D'Angelo, Edgardo ; Koutsoukou, Antonia ; Della Valle, Patrizia ; Gentile, Guendalina ; Pecchiari, Matteo. / Cytokine release, small airway injury, and parenchymal damage during mechanical ventilation in normal open-chest rats. In: Journal of Applied Physiology. 2008 ; Vol. 104, No. 1. pp. 41-49.
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