Cytokines and chemokines are both expressed by human myoblasts

Possible relevance for the immune pathogenesis of muscle inflammation

Marco De Rossi, Pia Bernasconi, Fulvio Baggi, Rene De Waal Malefyt, Renato Mantegazza

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

The idiopathic inflammatory myopathies are characterized by antibody- or cell-mediated immune response against unknown muscle tissue antigens. In these diseases a cellular infiltrate, composed of T and B lymphocytes, macrophages and NK cells, may invade muscle tissue with a gradient from the perivascular space to the endomysial compartment. Muscle cells may be actively involved in the processes of mononuclear cell recruitment and activation from the blood stream to the areas of inflammation. In order to verify this hypothesis, cultured human myoblasts were tested for their capacity to express different pro-inflammatory cytokines [IL-1α, IL-1β, IL-6 and tumor necrosis factor (TNF)-α] and chemokines (IL-8, MCP-1 and RANTES) at the mRNA level and protein secretion, in the presence of the pro-inflammatory cytokines IFN-γ and TNF-α alone or in combination. We confirmed that human myoblasts expressed IL-1α and IL-6 constitutively, while IL-1β and TNF-α are detected only after treatment with pro-inflammatory cytokines; moreover, we observed that TNF-α was expressed on an autocrine fashion by myoblasts. IL-8 and RANTES were expressed constitutively while MCP-1 after proper induction. These molecular data were further confirmed by specific ELISA in the supernatant from cultured myoblasts. Our results underline the importance of human myoblasts in the recruitment of leukocytes from the blood stream and, most probably, in the cross-talk between infiltrating inflammatory cells and muscle cells, creating the conditions for a chronic inflammation. Moreover, the capacity of muscle cells to behave as cells of the immune system has to be kept in mind, also in view of i.m. vaccination and use of molecular engineered myoblasts as vehicles in gene therapy.

Original languageEnglish
Pages (from-to)1329-1335
Number of pages7
JournalInternational Immunology
Volume12
Issue number9
Publication statusPublished - 2000

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Myoblasts
Chemokines
Cytokines
Inflammation
Interleukin-1
Muscles
Tumor Necrosis Factor-alpha
Muscle Cells
Chemokine CCL5
Interleukin-8
Interleukin-6
Myositis
Natural Killer Cells
Genetic Therapy
Immune System
Vaccination
Leukocytes
B-Lymphocytes
Enzyme-Linked Immunosorbent Assay
Macrophages

Keywords

  • Chemokines
  • Cytokines
  • Human myoblasts
  • Idiopathic inflammatory myopathies

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Cytokines and chemokines are both expressed by human myoblasts: Possible relevance for the immune pathogenesis of muscle inflammation",
abstract = "The idiopathic inflammatory myopathies are characterized by antibody- or cell-mediated immune response against unknown muscle tissue antigens. In these diseases a cellular infiltrate, composed of T and B lymphocytes, macrophages and NK cells, may invade muscle tissue with a gradient from the perivascular space to the endomysial compartment. Muscle cells may be actively involved in the processes of mononuclear cell recruitment and activation from the blood stream to the areas of inflammation. In order to verify this hypothesis, cultured human myoblasts were tested for their capacity to express different pro-inflammatory cytokines [IL-1α, IL-1β, IL-6 and tumor necrosis factor (TNF)-α] and chemokines (IL-8, MCP-1 and RANTES) at the mRNA level and protein secretion, in the presence of the pro-inflammatory cytokines IFN-γ and TNF-α alone or in combination. We confirmed that human myoblasts expressed IL-1α and IL-6 constitutively, while IL-1β and TNF-α are detected only after treatment with pro-inflammatory cytokines; moreover, we observed that TNF-α was expressed on an autocrine fashion by myoblasts. IL-8 and RANTES were expressed constitutively while MCP-1 after proper induction. These molecular data were further confirmed by specific ELISA in the supernatant from cultured myoblasts. Our results underline the importance of human myoblasts in the recruitment of leukocytes from the blood stream and, most probably, in the cross-talk between infiltrating inflammatory cells and muscle cells, creating the conditions for a chronic inflammation. Moreover, the capacity of muscle cells to behave as cells of the immune system has to be kept in mind, also in view of i.m. vaccination and use of molecular engineered myoblasts as vehicles in gene therapy.",
keywords = "Chemokines, Cytokines, Human myoblasts, Idiopathic inflammatory myopathies",
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T1 - Cytokines and chemokines are both expressed by human myoblasts

T2 - Possible relevance for the immune pathogenesis of muscle inflammation

AU - De Rossi, Marco

AU - Bernasconi, Pia

AU - Baggi, Fulvio

AU - De Waal Malefyt, Rene

AU - Mantegazza, Renato

PY - 2000

Y1 - 2000

N2 - The idiopathic inflammatory myopathies are characterized by antibody- or cell-mediated immune response against unknown muscle tissue antigens. In these diseases a cellular infiltrate, composed of T and B lymphocytes, macrophages and NK cells, may invade muscle tissue with a gradient from the perivascular space to the endomysial compartment. Muscle cells may be actively involved in the processes of mononuclear cell recruitment and activation from the blood stream to the areas of inflammation. In order to verify this hypothesis, cultured human myoblasts were tested for their capacity to express different pro-inflammatory cytokines [IL-1α, IL-1β, IL-6 and tumor necrosis factor (TNF)-α] and chemokines (IL-8, MCP-1 and RANTES) at the mRNA level and protein secretion, in the presence of the pro-inflammatory cytokines IFN-γ and TNF-α alone or in combination. We confirmed that human myoblasts expressed IL-1α and IL-6 constitutively, while IL-1β and TNF-α are detected only after treatment with pro-inflammatory cytokines; moreover, we observed that TNF-α was expressed on an autocrine fashion by myoblasts. IL-8 and RANTES were expressed constitutively while MCP-1 after proper induction. These molecular data were further confirmed by specific ELISA in the supernatant from cultured myoblasts. Our results underline the importance of human myoblasts in the recruitment of leukocytes from the blood stream and, most probably, in the cross-talk between infiltrating inflammatory cells and muscle cells, creating the conditions for a chronic inflammation. Moreover, the capacity of muscle cells to behave as cells of the immune system has to be kept in mind, also in view of i.m. vaccination and use of molecular engineered myoblasts as vehicles in gene therapy.

AB - The idiopathic inflammatory myopathies are characterized by antibody- or cell-mediated immune response against unknown muscle tissue antigens. In these diseases a cellular infiltrate, composed of T and B lymphocytes, macrophages and NK cells, may invade muscle tissue with a gradient from the perivascular space to the endomysial compartment. Muscle cells may be actively involved in the processes of mononuclear cell recruitment and activation from the blood stream to the areas of inflammation. In order to verify this hypothesis, cultured human myoblasts were tested for their capacity to express different pro-inflammatory cytokines [IL-1α, IL-1β, IL-6 and tumor necrosis factor (TNF)-α] and chemokines (IL-8, MCP-1 and RANTES) at the mRNA level and protein secretion, in the presence of the pro-inflammatory cytokines IFN-γ and TNF-α alone or in combination. We confirmed that human myoblasts expressed IL-1α and IL-6 constitutively, while IL-1β and TNF-α are detected only after treatment with pro-inflammatory cytokines; moreover, we observed that TNF-α was expressed on an autocrine fashion by myoblasts. IL-8 and RANTES were expressed constitutively while MCP-1 after proper induction. These molecular data were further confirmed by specific ELISA in the supernatant from cultured myoblasts. Our results underline the importance of human myoblasts in the recruitment of leukocytes from the blood stream and, most probably, in the cross-talk between infiltrating inflammatory cells and muscle cells, creating the conditions for a chronic inflammation. Moreover, the capacity of muscle cells to behave as cells of the immune system has to be kept in mind, also in view of i.m. vaccination and use of molecular engineered myoblasts as vehicles in gene therapy.

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