Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane

Peter Maier, Nicole Rathfelder, Martin G. Finkbeiner, Christof Taxis, Massimiliano Mazza, Sophie Le Panse, Rosine Haguenauer-Tsapis, Michael Knop

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Intracellular budding is a developmentally regulated type of cell division common to many fungi and protists. In Saccaromyces cerevisiae, intracellular budding requires the de novo assembly of membranes, the prospore membranes (PSMs) and occurs during spore formation in meiosis. Ssp1p is a sporulation-specific protein that has previously been shown to localize to secretory vesicles and to recruit the leading edge protein coat (LEP coat) proteins to the opening of the PSM. Here, we show that Ssp1p is a multidomain protein with distinct domains important for PI(4,5)P2 binding, binding to secretory vesicles and inhibition of vesicle fusion, interaction with LEP coat components and that it is subject to sumoylation and degradation. We found non-essential roles for Ssp1p on the level of vesicle transport and an essential function of Ssp1p to regulate the opening of the PSM. Together, our results indicate that Ssp1p has a domain architecture that resembles to some extent the septin class of proteins, and that the regulated removal of Ssp1p from the PSM is the major step underlying cytokinesis in yeast sporulation.

Original languageEnglish
Pages (from-to)1843-1852
Number of pages10
JournalEMBO Journal
Volume26
Issue number7
DOIs
Publication statusPublished - Apr 4 2007

Fingerprint

Cytokinesis
Meiosis
Yeast
Yeasts
Membranes
Secretory Vesicles
Capsid Proteins
Proteins
Septins
Sumoylation
Transport Vesicles
Spores
Fungi
Cell Division
Fusion reactions
Cells
Degradation

Keywords

  • Cytokinesis
  • Intracellular budding
  • Septins
  • Sporulation
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Maier, P., Rathfelder, N., Finkbeiner, M. G., Taxis, C., Mazza, M., Panse, S. L., ... Knop, M. (2007). Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane. EMBO Journal, 26(7), 1843-1852. https://doi.org/10.1038/sj.emboj.7601621

Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane. / Maier, Peter; Rathfelder, Nicole; Finkbeiner, Martin G.; Taxis, Christof; Mazza, Massimiliano; Panse, Sophie Le; Haguenauer-Tsapis, Rosine; Knop, Michael.

In: EMBO Journal, Vol. 26, No. 7, 04.04.2007, p. 1843-1852.

Research output: Contribution to journalArticle

Maier, P, Rathfelder, N, Finkbeiner, MG, Taxis, C, Mazza, M, Panse, SL, Haguenauer-Tsapis, R & Knop, M 2007, 'Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane', EMBO Journal, vol. 26, no. 7, pp. 1843-1852. https://doi.org/10.1038/sj.emboj.7601621
Maier P, Rathfelder N, Finkbeiner MG, Taxis C, Mazza M, Panse SL et al. Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane. EMBO Journal. 2007 Apr 4;26(7):1843-1852. https://doi.org/10.1038/sj.emboj.7601621
Maier, Peter ; Rathfelder, Nicole ; Finkbeiner, Martin G. ; Taxis, Christof ; Mazza, Massimiliano ; Panse, Sophie Le ; Haguenauer-Tsapis, Rosine ; Knop, Michael. / Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane. In: EMBO Journal. 2007 ; Vol. 26, No. 7. pp. 1843-1852.
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