Cytolytic activity of circulating human monocytes on transformed and untransformed human fibroblasts

A. Mantovani, A. Tagliabue, J. H. Dean, T. R. Jerrells, R. B. Herberman

Research output: Contribution to journalArticle

Abstract

Monocytes from the peripheral blood of 10 normal adult human donors were tested for their cytolytic activity on untransformed or SV40-transformed, early passage, human fibroblasts. Two pairs of human fibroblast lines (2303 and SV40-2303, 2931 and SV40-2931) were used and lysis was assessed in terms of release of [ 3H] thymidine from prelabelled target cells over a 72-h incubation period. High levels of cytolytic activity were consistently observed on transformed fibroblasts at attacker to target cell (A:T) ratios ranging from 5:1 to 40:1. Low, but significant, lysis of untransformed cells was observed only with two donors at the highest (40:1) A:T ratio tested. With all donors, the cytolytic activity on transformed lines was significantly greater than on untransformed fibroblasts. Thus natural human monocyte-mediated cytotoxicity is preferentially expressed on transformed, as compared to untransformed, cells although sparing of the latter is not absolute.

Original languageEnglish
Pages (from-to)28-31
Number of pages4
JournalInternational Journal of Cancer
Volume23
Issue number1
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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