TY - JOUR
T1 - Cytolytic function of clonable T cells after human bone marrow transplantation
AU - Velardi, Andrea
AU - Varese, Paola
AU - Grossi, Carlo E.
AU - Albi, Nicola
AU - Dembech, Chiara
AU - Terenzi, Adelmo
AU - Moretta, Lorenzo
AU - Grignani, Fausto
AU - Martelli, Massimo F.
AU - Mingari, Maria Cristina
PY - 1990/3/15
Y1 - 1990/3/15
N2 - We evaluated T-cell mediated lymphokine activated killer (LAK) function during the late (greater than 5 months) reconstitution phase after T cell-depleted allogeneic bone marrow transplantation (BMT) for hematologic malignancy. Since LAK cells are sustained by interleukin-2 (IL-2), we also investigated the ability of post-BMT T cells to produce IL-2. These functions were investigated at the clonal level. More than 200 T-cell clones from six long-term BMT recipients were generated and compared with 60 T-cell clones derived from two normal controls. Almost all the CD8+ clonal cultures from BMT recipients expressed cytolytic activity in a lectin-dependent cellular cytoxicity assay. Interestingly, a higher proportion of BMT recipientderived cytolytic clones were able to mediate LAK activity in comparison with control clones (28% versus 4%, P <.05) However, T-cell clones from BMT recipients, as opposed to control clones, were largely incapable of producing IL-2. Given the high proportions of post-BMT circulating CD8+ T cells, it appears that, in long-term BMT recipients, the precursors of nonspecific LAK effectors are present at above normal levels. However, their function may be defective in vivo due to poor IL-2 production.
AB - We evaluated T-cell mediated lymphokine activated killer (LAK) function during the late (greater than 5 months) reconstitution phase after T cell-depleted allogeneic bone marrow transplantation (BMT) for hematologic malignancy. Since LAK cells are sustained by interleukin-2 (IL-2), we also investigated the ability of post-BMT T cells to produce IL-2. These functions were investigated at the clonal level. More than 200 T-cell clones from six long-term BMT recipients were generated and compared with 60 T-cell clones derived from two normal controls. Almost all the CD8+ clonal cultures from BMT recipients expressed cytolytic activity in a lectin-dependent cellular cytoxicity assay. Interestingly, a higher proportion of BMT recipientderived cytolytic clones were able to mediate LAK activity in comparison with control clones (28% versus 4%, P <.05) However, T-cell clones from BMT recipients, as opposed to control clones, were largely incapable of producing IL-2. Given the high proportions of post-BMT circulating CD8+ T cells, it appears that, in long-term BMT recipients, the precursors of nonspecific LAK effectors are present at above normal levels. However, their function may be defective in vivo due to poor IL-2 production.
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M3 - Article
C2 - 2310833
AN - SCOPUS:0025250266
VL - 75
SP - 1364
EP - 1369
JO - Blood
JF - Blood
SN - 0006-4971
IS - 6
ER -