Cytolytic function of clonable T cells after human bone marrow transplantation

Andrea Velardi, Paola Varese, Carlo E. Grossi, Nicola Albi, Chiara Dembech, Adelmo Terenzi, Lorenzo Moretta, Fausto Grignani, Massimo F. Martelli, Maria Cristina Mingari

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated T-cell mediated lymphokine activated killer (LAK) function during the late (greater than 5 months) reconstitution phase after T cell-depleted allogeneic bone marrow transplantation (BMT) for hematologic malignancy. Since LAK cells are sustained by interleukin-2 (IL-2), we also investigated the ability of post-BMT T cells to produce IL-2. These functions were investigated at the clonal level. More than 200 T-cell clones from six long-term BMT recipients were generated and compared with 60 T-cell clones derived from two normal controls. Almost all the CD8+ clonal cultures from BMT recipients expressed cytolytic activity in a lectin-dependent cellular cytoxicity assay. Interestingly, a higher proportion of BMT recipientderived cytolytic clones were able to mediate LAK activity in comparison with control clones (28% versus 4%, P <.05) However, T-cell clones from BMT recipients, as opposed to control clones, were largely incapable of producing IL-2. Given the high proportions of post-BMT circulating CD8+ T cells, it appears that, in long-term BMT recipients, the precursors of nonspecific LAK effectors are present at above normal levels. However, their function may be defective in vivo due to poor IL-2 production.

Original languageEnglish
Pages (from-to)1364-1369
Number of pages6
JournalBlood
Volume75
Issue number6
Publication statusPublished - Mar 15 1990

ASJC Scopus subject areas

  • Hematology

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