CytoMatrix for a reliable and simple characterization of lung cancer stem cells from malignant pleural effusions

Sara Bruschini, Simona di Martino, Maria Elena Pisanu, Luigi Fattore, Claudia De Vitis, Valentina Laquintana, Simonetta Buglioni, Eugenio Tabbì, Andrea Cerri, Paolo Visca, Gabriele Alessandrini, Francesco Facciolo, Christian Napoli, Marcella Trombetta, Antonio Santoro, Anna Crescenzi, Gennaro Ciliberto, Rita Mancini

Research output: Contribution to journalArticle

Abstract

Cancer stem cells (CSCs) are a subpopulation with the properties of extensive self-renewal, capability to generate differentiated cancer cells and resistance to therapies. We have previously shown that malignant pleural effusions (MPEs) from patients with non-small-cell lung cancer (NSCLC) represent a valuable source of cancer cells that can be grown as three-dimensional (3D) spheroids enriched for stem-like features, which depend on the activation of the Yes-associated protein-transcriptional coactivator with PDZ-binding motif (YAP-TAZ)/Wnt-βcatenin/stearoyl-CoA desaturase 1 (SCD1) axis. Here, we describe a novel support, called CytoMatrix, for the characterization of limited amounts of cancer cells isolated from MPEs of patients with NSCLC. Our results show that this synthetic matrix allows an easy and fast characterization of several epithelial cellular markers. The use of CytoMatrix to study CSCs subpopulation confirms that SCD1 protein expression is enhanced in 3D spheroids when compared with 2D adherent cell cultures. YAP/TAZ nuclear-cytoplasmic distribution analysed by CytoMatrix in 3D spheroids is highly heterogeneous and faithfully reproduces what is observed in tumour biopsies. Our results confirm and extend the robustness of our workflow for the isolation and phenotypic characterization of primary cancer cells derived from the lung MPEs and underscore the role of SCD1.

Original languageEnglish
Pages (from-to)1877-1887
Number of pages11
JournalJournal of Cellular Physiology
Volume235
Issue number3
DOIs
Publication statusPublished - Mar 1 2020

Fingerprint

Malignant Pleural Effusion
Neoplastic Stem Cells
Stem cells
Stearoyl-CoA Desaturase
Lung Neoplasms
Cells
Neoplasms
Non-Small Cell Lung Carcinoma
Catenins
Workflow
Biopsy
Cell culture
Tumors
Proteins
Cell Culture Techniques
Chemical activation
Lung

Keywords

  • cancer stem cells
  • CytoMatrix
  • lung cancer
  • MPEs
  • SCD1

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

CytoMatrix for a reliable and simple characterization of lung cancer stem cells from malignant pleural effusions. / Bruschini, Sara; di Martino, Simona; Pisanu, Maria Elena; Fattore, Luigi; De Vitis, Claudia; Laquintana, Valentina; Buglioni, Simonetta; Tabbì, Eugenio; Cerri, Andrea; Visca, Paolo; Alessandrini, Gabriele; Facciolo, Francesco; Napoli, Christian; Trombetta, Marcella; Santoro, Antonio; Crescenzi, Anna; Ciliberto, Gennaro; Mancini, Rita.

In: Journal of Cellular Physiology, Vol. 235, No. 3, 01.03.2020, p. 1877-1887.

Research output: Contribution to journalArticle

Bruschini, Sara ; di Martino, Simona ; Pisanu, Maria Elena ; Fattore, Luigi ; De Vitis, Claudia ; Laquintana, Valentina ; Buglioni, Simonetta ; Tabbì, Eugenio ; Cerri, Andrea ; Visca, Paolo ; Alessandrini, Gabriele ; Facciolo, Francesco ; Napoli, Christian ; Trombetta, Marcella ; Santoro, Antonio ; Crescenzi, Anna ; Ciliberto, Gennaro ; Mancini, Rita. / CytoMatrix for a reliable and simple characterization of lung cancer stem cells from malignant pleural effusions. In: Journal of Cellular Physiology. 2020 ; Vol. 235, No. 3. pp. 1877-1887.
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