Cytometry by time of flight identifies distinct signatures in patients with systemic sclerosis, systemic lupus erythematosus and Sjögrens syndrome

Maarten van der Kroef, Lucas L. van den Hoogen, Jorre S. Mertens, Sofie L.M. Blokland, Scott Haskett, Abhinandan Devaprasad, Tiago Carvalheiro, Eleni Chouri, Nadia Vazirpanah, Marta Cossu, Catherina G.K. Wichers, Sandra C. Silva-Cardoso, Alsya J. Affandi, Cornelis P.J. Bekker, Ana P. Lopes, Maarten R. Hillen, Femke Bonte-Mineur, Marc R. Kok, Lorenzo Beretta, Marzia RossatoMichaël Mingueneau, Joel A.G. van Roon, Timothy R.D.J. Radstake

Research output: Contribution to journalArticle

Abstract

Systemic sclerosis (SSc), systemic lupus erythematosus (SLE) and primary Sjögrens syndrome (pSS) are clinically distinct systemic autoimmune diseases (SADs) that share molecular pathways. We quantified the frequency of circulating immune-cells in 169 patients with these SADs and 44 healty controls (HC) using mass-cytometry and assessed the diagnostic value of these results. Alterations in the frequency of immune-cell subsets were present in all SADs compared to HC. Most alterations, including a decrease of CD56hi NK-cells in SSc and IgM+ Bcells in pSS, were disease specific; only a reduced frequency of plasmacytoid dendritic cells was common between all SADs Strikingly, hierarchical clustering of SSc patients identified 4 clusters associated with different clinical phenotypes, and 9 of the 12 cell subset-alterations in SSc were also present during the preclinical-phase of the disease. Additionally, we found a strong association between the use of prednisone and alterations in B-cell subsets. Although differences in immune-cell frequencies between these SADs are apparent, the discriminative value thereof is too low for diagnostic purposes. Within each disease, mass cytometry analyses revealed distinct patterns between endophenotypes. Given the lack of tools enabling early diagnosis of SSc, our results justify further research into the value of cellular phenotyping as a diagnostic aid.

Original languageEnglish
Pages (from-to)119-129
JournalEuropean Journal of Immunology
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 1 2020

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Systemic Scleroderma
Systemic Lupus Erythematosus
Autoimmune Diseases
Endophenotypes
B-Lymphocyte Subsets
Prednisone
Natural Killer Cells
Dendritic Cells
Immunoglobulin M
Cluster Analysis
Early Diagnosis
Phenotype
Research

Keywords

  • CyTOF
  • immunophenotyping
  • Sjögren's syndrome
  • systemic lupus erythematosus
  • systemic sclerosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

van der Kroef, M., van den Hoogen, L. L., Mertens, J. S., Blokland, S. L. M., Haskett, S., Devaprasad, A., ... Radstake, T. R. D. J. (2020). Cytometry by time of flight identifies distinct signatures in patients with systemic sclerosis, systemic lupus erythematosus and Sjögrens syndrome. European Journal of Immunology, 50(1), 119-129. https://doi.org/10.1002/eji.201948129

Cytometry by time of flight identifies distinct signatures in patients with systemic sclerosis, systemic lupus erythematosus and Sjögrens syndrome. / van der Kroef, Maarten; van den Hoogen, Lucas L.; Mertens, Jorre S.; Blokland, Sofie L.M.; Haskett, Scott; Devaprasad, Abhinandan; Carvalheiro, Tiago; Chouri, Eleni; Vazirpanah, Nadia; Cossu, Marta; Wichers, Catherina G.K.; Silva-Cardoso, Sandra C.; Affandi, Alsya J.; Bekker, Cornelis P.J.; Lopes, Ana P.; Hillen, Maarten R.; Bonte-Mineur, Femke; Kok, Marc R.; Beretta, Lorenzo; Rossato, Marzia; Mingueneau, Michaël; van Roon, Joel A.G.; Radstake, Timothy R.D.J.

In: European Journal of Immunology, Vol. 50, No. 1, 01.01.2020, p. 119-129.

Research output: Contribution to journalArticle

van der Kroef, M, van den Hoogen, LL, Mertens, JS, Blokland, SLM, Haskett, S, Devaprasad, A, Carvalheiro, T, Chouri, E, Vazirpanah, N, Cossu, M, Wichers, CGK, Silva-Cardoso, SC, Affandi, AJ, Bekker, CPJ, Lopes, AP, Hillen, MR, Bonte-Mineur, F, Kok, MR, Beretta, L, Rossato, M, Mingueneau, M, van Roon, JAG & Radstake, TRDJ 2020, 'Cytometry by time of flight identifies distinct signatures in patients with systemic sclerosis, systemic lupus erythematosus and Sjögrens syndrome', European Journal of Immunology, vol. 50, no. 1, pp. 119-129. https://doi.org/10.1002/eji.201948129
van der Kroef, Maarten ; van den Hoogen, Lucas L. ; Mertens, Jorre S. ; Blokland, Sofie L.M. ; Haskett, Scott ; Devaprasad, Abhinandan ; Carvalheiro, Tiago ; Chouri, Eleni ; Vazirpanah, Nadia ; Cossu, Marta ; Wichers, Catherina G.K. ; Silva-Cardoso, Sandra C. ; Affandi, Alsya J. ; Bekker, Cornelis P.J. ; Lopes, Ana P. ; Hillen, Maarten R. ; Bonte-Mineur, Femke ; Kok, Marc R. ; Beretta, Lorenzo ; Rossato, Marzia ; Mingueneau, Michaël ; van Roon, Joel A.G. ; Radstake, Timothy R.D.J. / Cytometry by time of flight identifies distinct signatures in patients with systemic sclerosis, systemic lupus erythematosus and Sjögrens syndrome. In: European Journal of Immunology. 2020 ; Vol. 50, No. 1. pp. 119-129.
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AU - van der Kroef, Maarten

AU - van den Hoogen, Lucas L.

AU - Mertens, Jorre S.

AU - Blokland, Sofie L.M.

AU - Haskett, Scott

AU - Devaprasad, Abhinandan

AU - Carvalheiro, Tiago

AU - Chouri, Eleni

AU - Vazirpanah, Nadia

AU - Cossu, Marta

AU - Wichers, Catherina G.K.

AU - Silva-Cardoso, Sandra C.

AU - Affandi, Alsya J.

AU - Bekker, Cornelis P.J.

AU - Lopes, Ana P.

AU - Hillen, Maarten R.

AU - Bonte-Mineur, Femke

AU - Kok, Marc R.

AU - Beretta, Lorenzo

AU - Rossato, Marzia

AU - Mingueneau, Michaël

AU - van Roon, Joel A.G.

AU - Radstake, Timothy R.D.J.

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