Cytophilic antibody: correlation of its distribution with activation of basophils and macrophages

M. Ferrarini, A. Munro, A. B. Wilson

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Immunoglubulin determinants on the surface of guinea pig basophils were detected using mono- and divalent antiglobulin reagents conjugated with fluorescein isothiocyanate. The fluorescent pattern obtained with monovalent antiglobulin was always that of uniformly stained rings. Divalent antiglobulin agglutinated immunoglobulin determinants to form small fluorescent spots subsequently collected at one end of the cell ('cap' formation). The formation of caps is temperature dependent and is inhibited by sodium azide and cytochalasin B. When basophils were incubated at 37°C with divalent antiglobulin, morphological changes occurred, indicating that they had been activated. The same effect could be obtained with monovalent Fab', but at least 100 times more of it was required than the corresponding F(ab')2. Cytophilic antibodies on the surface of guinea pig peritoneal macrophages were also detected by immunofluorescence. In this case again, divalent antiglobulin reagents agglutinated the determinants to form small fluorescent spots. Cap formation was not observed and pinocytosis started as the first aggregates were formed. It was also that cytophilic antibodies were not stable on the macrophages' surface and were easily eluted at 37°C. A reaction with cross linking agents (divalent antiglobulin or antigen) stabilized the cytophilic antibody and pinocytosis was initiated.

Original languageEnglish
Pages (from-to)364-370
Number of pages7
JournalEuropean Journal of Immunology
Issue number6
Publication statusPublished - 1973

ASJC Scopus subject areas

  • Immunology


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