Cytoskeleton mediates negative inotropism and lusitropism of chromogranin A-derived peptides (human vasostatin1-78 and rat CgA1-64) in the rat heart

Tommaso Angelone, Anna Maria Quintieri, Yannich Goumon, Valentina Di Felice, Elisabetta Filice, Alfonsina Gattuso, Rosa Mazza, Angelo Corti, Bruno Tota, Marie Hélène Metz-Boutigue, Maria Carmela Cerra

Research output: Contribution to journalArticle

Abstract

Cytoskeleton scaffold in cardiac myocytes provides structural support and compartmentalization of intracellular components. It is implicated in cardiac pathologies including hypertrophy and failure, playing a key role in the determinism of contractile and diastolic dysfunctions. Chromogranin A (CgA) and its derived peptides have revealed themselves as novel cardiovascular modulators. In humans, normal CgA levels considerably increase in several pathologies, including heart failure. Recent data have shown on the unstimulated rat heart that human recombinant Vasostatin-1 (hrVS-1) and rat chromogranin A 1-64 (rCgA1-64) induce negative inotropic and lusitropic effects counteracting the β-adrenergic-dependent positive inotropism with a functional non-competitive antagonism. This study investigates, on the isolated Langendorff perfused rat heart, whether cardiac cytoskeleton is involved in the modulation of contractility and relaxation exerted by hrVS-1 and rCgA1-64. Cytoskeleton impairment by either cytochalasin-D (actin polymerization inhibitor), BDM (myosin ATP-ase antagonist) or wortmannin (inhibitor of PI3-K/Akt transduction cascade), or W-7 (calcium-calmodulin antagonist) abolished hrVS-1 and rCgA1-64-mediated inotropism and lusitropism. Using fluorescent phalloidin, we showed on rat cardiac H9C2 cells that hrVS-1 (10nM÷10μM) stimulates actin polymerization. Taken together these data indicate that in the rat heart, the actin cytoskeletal network strongly contributes to the cardiotropic action of CgA-derived peptides.

Original languageEnglish
Pages (from-to)78-85
Number of pages8
JournalRegulatory Peptides
Volume165
Issue number1
DOIs
Publication statusPublished - Nov 30 2010

Keywords

  • Cardiomyocytes
  • Cytoskeleton
  • Inotropy
  • Lusitropy
  • Rat CgA
  • Rat Langendorff heart
  • Vasostatin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Angelone, T., Quintieri, A. M., Goumon, Y., Di Felice, V., Filice, E., Gattuso, A., Mazza, R., Corti, A., Tota, B., Metz-Boutigue, M. H., & Cerra, M. C. (2010). Cytoskeleton mediates negative inotropism and lusitropism of chromogranin A-derived peptides (human vasostatin1-78 and rat CgA1-64) in the rat heart. Regulatory Peptides, 165(1), 78-85. https://doi.org/10.1016/j.regpep.2009.10.003