Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model

Ersilia Dolfini, Leda Roncoroni, Luca Elli, Chiara Fumagalli, Roberto Colombo, Simona Ramponi, Fabio Forlani, Maria Teresa Bardella

Research output: Contribution to journalArticle

Abstract

Aim: To evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns. Methods: We treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence) and the tight-junction protein occludin and zonula occluden-1. Results: The treated spheroids had deep holes and surface b lebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin filaments, impaired and disassembled the integrity of the tight-junction system. Conclusion: Our data obtained from an "in vivolike" polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions.

Original languageEnglish
Pages (from-to)7597-7601
Number of pages5
JournalWorld Journal of Gastroenterology
Volume11
Issue number48
Publication statusPublished - Dec 28 2005

Fingerprint

Gliadin
Cytoskeleton
Tight Junctions
Cellular Spheroids
Occludin
Tight Junction Proteins
Phalloidine
Bread
Actin Cytoskeleton
Cell Communication
Electron Scanning Microscopy
Triticum
In Vitro Techniques
Actins
Microscopy
Cell Culture Techniques
Fluorescence
Epithelial Cells
Light

Keywords

  • Celiac disease
  • Cytoskeleton
  • Gliadin
  • Multicellular spheroids

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model. / Dolfini, Ersilia; Roncoroni, Leda; Elli, Luca; Fumagalli, Chiara; Colombo, Roberto; Ramponi, Simona; Forlani, Fabio; Bardella, Maria Teresa.

In: World Journal of Gastroenterology, Vol. 11, No. 48, 28.12.2005, p. 7597-7601.

Research output: Contribution to journalArticle

Dolfini, E, Roncoroni, L, Elli, L, Fumagalli, C, Colombo, R, Ramponi, S, Forlani, F & Bardella, MT 2005, 'Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model', World Journal of Gastroenterology, vol. 11, no. 48, pp. 7597-7601.
Dolfini, Ersilia ; Roncoroni, Leda ; Elli, Luca ; Fumagalli, Chiara ; Colombo, Roberto ; Ramponi, Simona ; Forlani, Fabio ; Bardella, Maria Teresa. / Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 48. pp. 7597-7601.
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