Cytosol cathepsin-D content and proliferative activity of human breast cancer

Angelo Paradiso, Anita Mangia, Mario Correale, Ines Abbate, Giovanni Ferri, Adriano Piffanelli, Laura Catozzi, Dino Amadori, Angela Riccobon, Angela Riccobon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBreast Cancer Research and Treatment
Volume23
Issue number1-2
DOIs
Publication statusPublished - Feb 1992

Fingerprint

Cathepsin D
Human Activities
Cytosol
Breast Neoplasms
Thymidine
Immunoradiometric Assay
S Phase
Neoplasms
Peptide Hydrolases
Hormones
Proteins

Keywords

  • breast cancer
  • cathepsin-D
  • proliferative activity

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cytosol cathepsin-D content and proliferative activity of human breast cancer. / Paradiso, Angelo; Mangia, Anita; Correale, Mario; Abbate, Ines; Ferri, Giovanni; Piffanelli, Adriano; Catozzi, Laura; Amadori, Dino; Riccobon, Angela; Riccobon, Angela.

In: Breast Cancer Research and Treatment, Vol. 23, No. 1-2, 02.1992, p. 63-70.

Research output: Contribution to journalArticle

Paradiso, A, Mangia, A, Correale, M, Abbate, I, Ferri, G, Piffanelli, A, Catozzi, L, Amadori, D, Riccobon, A & Riccobon, A 1992, 'Cytosol cathepsin-D content and proliferative activity of human breast cancer', Breast Cancer Research and Treatment, vol. 23, no. 1-2, pp. 63-70. https://doi.org/10.1007/BF01831477
Paradiso, Angelo ; Mangia, Anita ; Correale, Mario ; Abbate, Ines ; Ferri, Giovanni ; Piffanelli, Adriano ; Catozzi, Laura ; Amadori, Dino ; Riccobon, Angela ; Riccobon, Angela. / Cytosol cathepsin-D content and proliferative activity of human breast cancer. In: Breast Cancer Research and Treatment. 1992 ; Vol. 23, No. 1-2. pp. 63-70.
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