Abstract
Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p
Original language | English |
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Pages (from-to) | 63-70 |
Number of pages | 8 |
Journal | Breast Cancer Research and Treatment |
Volume | 23 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Feb 1992 |
Keywords
- breast cancer
- cathepsin-D
- proliferative activity
ASJC Scopus subject areas
- Oncology
- Cancer Research