Abstract
Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p
| Original language | English |
|---|---|
| Pages (from-to) | 63-70 |
| Number of pages | 8 |
| Journal | Breast Cancer Research and Treatment |
| Volume | 23 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Feb 1992 |
Fingerprint
Keywords
- breast cancer
- cathepsin-D
- proliferative activity
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Cytosol cathepsin-D content and proliferative activity of human breast cancer. / Paradiso, Angelo; Mangia, Anita; Correale, Mario; Abbate, Ines; Ferri, Giovanni; Piffanelli, Adriano; Catozzi, Laura; Amadori, Dino; Riccobon, Angela; Riccobon, Angela.
In: Breast Cancer Research and Treatment, Vol. 23, No. 1-2, 02.1992, p. 63-70.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytosol cathepsin-D content and proliferative activity of human breast cancer
AU - Paradiso, Angelo
AU - Mangia, Anita
AU - Correale, Mario
AU - Abbate, Ines
AU - Ferri, Giovanni
AU - Piffanelli, Adriano
AU - Catozzi, Laura
AU - Amadori, Dino
AU - Riccobon, Angela
AU - Riccobon, Angela
PY - 1992/2
Y1 - 1992/2
N2 - Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p
AB - Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p
KW - breast cancer
KW - cathepsin-D
KW - proliferative activity
UR - http://www.scopus.com/inward/record.url?scp=0026526279&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026526279&partnerID=8YFLogxK
U2 - 10.1007/BF01831477
DO - 10.1007/BF01831477
M3 - Article
VL - 23
SP - 63
EP - 70
JO - Breast Cancer Research and Treatment
JF - Breast Cancer Research and Treatment
SN - 0167-6806
IS - 1-2
ER -