Cytosol cathepsin-D content and proliferative activity of human breast cancer

Angelo Paradiso, Anita Mangia, Mario Correale, Ines Abbate, Giovanni Ferri, Adriano Piffanelli, Laura Catozzi, Dino Amadori, Angela Riccobon, Angela Riccobon

Research output: Contribution to journalArticlepeer-review


Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBreast Cancer Research and Treatment
Issue number1-2
Publication statusPublished - Feb 1992


  • breast cancer
  • cathepsin-D
  • proliferative activity

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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