Cytosol cathepsin-D content and proliferative activity of human breast cancer

Angelo Paradiso; Anita Mangia; Mario Correale; Ines Abbate; Giovanni Ferri; Adriano Piffanelli; Laura Catozzi; Dino Amadori; Angela Riccobon; Angela Riccobon

doi:10.1007/BF01831477

Cytosol cathepsin-D content and proliferative activity of human breast cancer

Angelo Paradiso, Anita Mangia, Mario Correale, Ines Abbate, Giovanni Ferri, Adriano Piffanelli, Laura Catozzi, Dino Amadori, Angela Riccobon, Angela Riccobon

Research output: Contribution to journal › Article › peer-review

Abstract

Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p

Original language	English
Pages (from-to)	63-70
Number of pages	8
Journal	Breast Cancer Research and Treatment
Volume	23
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01831477
Publication status	Published - Feb 1992

Keywords

breast cancer
cathepsin-D
proliferative activity

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1007/BF01831477

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Cytosol cathepsin-D content and proliferative activity of human breast cancer. / Paradiso, Angelo ; Mangia, Anita; Correale, Mario; Abbate, Ines; Ferri, Giovanni; Piffanelli, Adriano; Catozzi, Laura; Amadori, Dino; Riccobon, Angela; Riccobon, Angela.

In: Breast Cancer Research and Treatment, Vol. 23, No. 1-2, 02.1992, p. 63-70.

Research output: Contribution to journal › Article › peer-review

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abstract = "Mitogenic properties have been demonstrated in vitro for the lysosomal acidic protease cathepsin-D (cath-D). We investigated possible relationships between cath-D cytosol cell content and tumor proliferative activity in a series of 129 operable breast cancer patients. For total cytosol cath-D evaluation, a solid phase two-site immunoradiometric assay was utilized on tumor cell cytosol obtained for hormone receptor assay (DCC method). The percentage of S-phase cells was analyzed by 3H-thymidine autoradiographic assay. Median 3H-thymidine Labeling Index (3H-Tdr-LI) of the series was 2.7%; median cath-D content resulted 57 pmol/mg of protein cytosol and was significantly higher in node-positive with respect to the node-negative subgroup (p",

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AU - Paradiso, Angelo

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AU - Abbate, Ines

AU - Ferri, Giovanni

AU - Piffanelli, Adriano

AU - Catozzi, Laura

AU - Amadori, Dino

AU - Riccobon, Angela

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Cytosol cathepsin-D content and proliferative activity of human breast cancer

Abstract

Keywords

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