Cytosolic and nuclear spermidine acetyltransferases in growing NIH 3T3 fibroblasts stimulated with serum or polyamines: Relationship to polyamine-biosynthetic decarboxylases and histone acetyltransferase

M. A. Desiderio, S. Mattei, G. Biondi, M. P. Colombo

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24 Citations (Scopus)

Abstract

The expression (mRNA level or enzymic activity) of cytosolic and nuclear spermidine acetyltransferases was studied in NIH 3T3 fibroblasts, either (1) serum-starved and stimulated to grow by serum refeeding, or (2) treated with inhibitors of ornithine decarboxylase (ODC) (MDL 72.175) and S-adenosylmethionine decarboxylase (AdoMetDC) (MDL 73.811) and stimulated to grow by spermidine. Expression of the known growth-regulated genes for ODC, AdoMetDC and histone acetyltransferase was also examined. The mRNA for spermidine/spermine N1-acetyl-transferase (SAT) accumulated after serum refeeding (between 6 and 16 h)and even more after spermidine addition(16 h). Histone acetyltransferase activity increased after both growth stimuli whereas spermidine N8-acetyltransferase activity remained unchanged. After serum stimulation, the ODC mRNA level and activity rose between 6 and 16 h, whereas AdoMetDC mRNA accumulation occurred later (16 h) than the increase in enzyme activity (6 h). Stimulation of ODC and AdoMetDC activities was suppressed by the inhibitors added alone or in combination with spermidine, whereas mRNA accumulation was down-regulated by spermidine. These results indicate that the expression of SAT was growth-controlled and that SAT mRNA level was regulated by polyamines.

Original languageEnglish
Pages (from-to)475-479
Number of pages5
JournalBiochemical Journal
Volume293
Issue number2
Publication statusPublished - 1993

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Histone Acetyltransferases
Carboxy-Lyases
Polyamines
Fibroblasts
Adenosylmethionine Decarboxylase
Spermidine
Messenger RNA
Ornithine Decarboxylase
Spermine
Transferases
Serum
Growth
Enzyme activity
diamine N-acetyltransferase
Genes
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "Cytosolic and nuclear spermidine acetyltransferases in growing NIH 3T3 fibroblasts stimulated with serum or polyamines: Relationship to polyamine-biosynthetic decarboxylases and histone acetyltransferase",
abstract = "The expression (mRNA level or enzymic activity) of cytosolic and nuclear spermidine acetyltransferases was studied in NIH 3T3 fibroblasts, either (1) serum-starved and stimulated to grow by serum refeeding, or (2) treated with inhibitors of ornithine decarboxylase (ODC) (MDL 72.175) and S-adenosylmethionine decarboxylase (AdoMetDC) (MDL 73.811) and stimulated to grow by spermidine. Expression of the known growth-regulated genes for ODC, AdoMetDC and histone acetyltransferase was also examined. The mRNA for spermidine/spermine N1-acetyl-transferase (SAT) accumulated after serum refeeding (between 6 and 16 h)and even more after spermidine addition(16 h). Histone acetyltransferase activity increased after both growth stimuli whereas spermidine N8-acetyltransferase activity remained unchanged. After serum stimulation, the ODC mRNA level and activity rose between 6 and 16 h, whereas AdoMetDC mRNA accumulation occurred later (16 h) than the increase in enzyme activity (6 h). Stimulation of ODC and AdoMetDC activities was suppressed by the inhibitors added alone or in combination with spermidine, whereas mRNA accumulation was down-regulated by spermidine. These results indicate that the expression of SAT was growth-controlled and that SAT mRNA level was regulated by polyamines.",
author = "Desiderio, {M. A.} and S. Mattei and G. Biondi and Colombo, {M. P.}",
year = "1993",
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journal = "Biochemical Journal",
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T1 - Cytosolic and nuclear spermidine acetyltransferases in growing NIH 3T3 fibroblasts stimulated with serum or polyamines

T2 - Relationship to polyamine-biosynthetic decarboxylases and histone acetyltransferase

AU - Desiderio, M. A.

AU - Mattei, S.

AU - Biondi, G.

AU - Colombo, M. P.

PY - 1993

Y1 - 1993

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