Cytosolic free calcium concentration in the mitogenic stimulation of T lymphocytes by anti-CD3 monoclonal antibodies

M. Murgia, M. Mion, L. Veronese, M. Panozzo, V. Coppola, R. Rizzuto, M. Brini, F. Malavasi, A. Amadori, L. Chieco Bianchi, T. Pozzan

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Abstract

The effects of anti-CD3 monoclonal antibodies on cytosolic free Ca2+ concentration, [Ca2+]i, were investigated in freshly isolated lymphocytes, T cell lines, T clones and the leukemic T cell line Jurkat with three different methodologies, i.e. classical cuvette experiments, cytofluorimetry and videoimaging. With any technique, concentrations of anti-CD3 antibodies optimal for stimulation of DNA synthesis were completely ineffective at inducing early increases of [Ca2+]i in freshly isolated lymphocytes. At supraoptimal mitogenic concentrations: (i) anti-CD3 mAb induced negligible increases of [Ca2+]i when tested in suspensions of freshly isolated lymphocytes, but the response increased progressively during in vitro culturing with IL2; (ii) most, but not all, T clones, when tested in suspension, were responsive to these concentrations of anti-CD3 antibodies in terms of [Ca2+]i; (iii) using the videoimaging technique at the single cell level, it was demonstrated that the anti-CD3 antibodies induced large increases of [Ca2+]i in lymphocytes only under conditions which allowed adherence of the antibodies (and of the cells) to the glass surface. In all T cell types investigated, the [Ca2+]i increases were most often composed by multiple, asynchronous oscillations. The buffering of [Ca2+]i increases, obtained by loading the cells with membrane permeant esters of Quin-2 and Fura-2, inhibited anti-CD3 mAb induced DNA synthesis, but this appeared entirely attributable to a toxic side effect of the ester hydrolysis. The relevance of these data is discussed in terms of their methodological and functional implications for the understanding of the role of Ca2+ in mitogenic stimulation of T cells.

Original languageEnglish
Pages (from-to)167-180
Number of pages14
JournalCell Calcium
Volume16
Issue number3
DOIs
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

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    Murgia, M., Mion, M., Veronese, L., Panozzo, M., Coppola, V., Rizzuto, R., Brini, M., Malavasi, F., Amadori, A., Chieco Bianchi, L., & Pozzan, T. (1994). Cytosolic free calcium concentration in the mitogenic stimulation of T lymphocytes by anti-CD3 monoclonal antibodies. Cell Calcium, 16(3), 167-180. https://doi.org/10.1016/0143-4160(94)90020-5