Cytosolic gangliosides of rat brain: Their fractionation into protein-bound complexes of different ganglioside compositions

S. Sonnino, R. Ghidoni, A. Fiorilli, B. Venerando, G. Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

The sialic acid moiety of rat brain cytosolic gangliosides was radiolabeled by intracranial injection of N-(3H)acetylmannosamine. Upon ammonium sulphate fractionation, Sepharose 6B gel filtration, and hydroxylapatite-cellulose chromatography, ganglioside-bound radioactivity of brain cytosolic extract followed the behavior of protein and not that of purified gangliosides. This indicates that cytosolic gangliosides occur as ganglioside-protein complexes. By application of hydroxylapatite-cellulose column chromatography, fractions were obtained having different ganglioside composition. In particular, one fraction contained GM1, one GD1a, and one GT1b with a ganglioside homogeneity better than 95% in each fraction. This indicates the occurrence in brain cytosol of a GM1-protein complex, a GD1a-protein complex, and a GT1b-protein complex.

Original languageEnglish
Pages (from-to)193-204
Number of pages12
JournalJournal of Neuroscience Research
Volume12
Issue number2-3
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Neuroscience(all)

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