Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle's disease

Graham J. Kemp, Caterina Tonon, Emil Malucelli, Claudia Testa, Alexandra Liava, David Manners, Enrico Trevisi, Andrea Martinuzzi, Bruno Barbiroli, Raffaele Lodi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cellular pH control is important in muscle physiology, and for interpretation of 31P magnetic resonance spectroscopy (MRS) data. Cellular acidification in exercise results from coupled glycolytic ATP production mitigated by cytosolic buffering, 'consumption' of H+ by phosphocreatine (PCr) breakdown, and membrane transport processes. Ex vivo methods for cytosolic buffer capacity are vulnerable to artefact, and MRS methods often require assumptions. 31P MRS of early exercise, when pH increases unopposed by glycolysis, is conceptually simple, but limited in normal muscle by time resolution and signal-to-noise. A therapeutic trial (Martinuzzi A et al. Musc Nerve 37: 350-357, 2007) in McArdle's disease (glycogen phosphorylase deficiency), where pH does not decrease with exercise, offered the opportunity to test 31P MRS data obtained throughout incremental plantar flexion exercise and recovery in ten McArdle's patients against the simple model of cellular pH control. Changes in pH, [Pi] and [PCr] throughout exercise and recovery were quantitatively consistent with mean ± SEM buffer capacity of 10 ± 1 mM/(pH unit), which was not significantly different from the control subjects under the initial-exercise conditions where the comparison could be made. The simple model of cellular acid-base balance therefore gives an adequate account of cellular pH changes during both exercise and recovery in McArdle's disease.

Original languageEnglish
Pages (from-to)687-694
Number of pages8
JournalEuropean Journal of Applied Physiology
Volume105
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Glycogen Storage Disease Type V
Skeletal Muscle
Exercise
Magnetic Resonance Spectroscopy
Phosphocreatine
Buffers
Glycogen Phosphorylase
Muscles
Acid-Base Equilibrium
Glycolysis
Artifacts
Noise
Adenosine Triphosphate
Membranes

Keywords

  • Acid-base
  • Buffer capacity
  • Buffering
  • McArdle's disease
  • Muscle glycogenosis
  • Muscle pH

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Orthopedics and Sports Medicine
  • Physiology (medical)

Cite this

Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle's disease. / Kemp, Graham J.; Tonon, Caterina; Malucelli, Emil; Testa, Claudia; Liava, Alexandra; Manners, David; Trevisi, Enrico; Martinuzzi, Andrea; Barbiroli, Bruno; Lodi, Raffaele.

In: European Journal of Applied Physiology, Vol. 105, No. 5, 2009, p. 687-694.

Research output: Contribution to journalArticle

Kemp, GJ, Tonon, C, Malucelli, E, Testa, C, Liava, A, Manners, D, Trevisi, E, Martinuzzi, A, Barbiroli, B & Lodi, R 2009, 'Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle's disease', European Journal of Applied Physiology, vol. 105, no. 5, pp. 687-694. https://doi.org/10.1007/s00421-008-0950-0
Kemp, Graham J. ; Tonon, Caterina ; Malucelli, Emil ; Testa, Claudia ; Liava, Alexandra ; Manners, David ; Trevisi, Enrico ; Martinuzzi, Andrea ; Barbiroli, Bruno ; Lodi, Raffaele. / Cytosolic pH buffering during exercise and recovery in skeletal muscle of patients with McArdle's disease. In: European Journal of Applied Physiology. 2009 ; Vol. 105, No. 5. pp. 687-694.
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