Cytotoxic activity in children with insulin-dependent diabetes mellitus

R. Lorini, A. Moretta, A. Valtorta, G. d'Annunzio, L. Cortona, L. Vitali, M. Bozzola, F. Severi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We determined the percentage of circulating natural killer (NK) cells, using the monoclonal antibodies anti-CD57 and anti-CD16, NK cytotoxic activity (lytic units/106) and lymphokine-activated killer (LAK) activity in 25 IDDM patients aged 3-23 years, 12 with disease for <1 year (Group I) and 13 with disease for > 3 years (Group II). Nine age-matched healthy subjects served as controls. The percentage of CD57+ cells was similar in IDDM patients and controls, while the percentage of CD16+ cells was lower in IDDM patients (P <0.05) than in controls. NK cell cytotoxic activity was lower in IDDM patients than in controls (P <0.01), in Group I and II compared with controls (P <0.005). LAK activity was similar in IDDM patients and in controls. No correlation was found between NK cytotoxic activity and metabolic control, HLA typing, while a negative correlation was found between NK cytotoxic activity and insulin requirement (P <0.05). The decreased NK cytotoxic activity observed in our patients, in particular in long-standing diabetics, with normal NK cell number, could be due to a qualitative defect of the NK cells, or to a deficient IL-2 and/or TNF-α production, or to a immunomodulatory or immunosuppressing effect of insulin.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalDiabetes Research and Clinical Practice
Volume23
Issue number1
DOIs
Publication statusPublished - 1994

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Type 1 Diabetes Mellitus
Natural Killer Cells
Lymphokines
Insulin
Histocompatibility Testing
Interleukin-2
Healthy Volunteers
Cell Count
Monoclonal Antibodies

Keywords

  • Insulin-dependent diabetes mellitus
  • Lymphokine-activated killer cells
  • Natural killer cells

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Cytotoxic activity in children with insulin-dependent diabetes mellitus. / Lorini, R.; Moretta, A.; Valtorta, A.; d'Annunzio, G.; Cortona, L.; Vitali, L.; Bozzola, M.; Severi, F.

In: Diabetes Research and Clinical Practice, Vol. 23, No. 1, 1994, p. 37-42.

Research output: Contribution to journalArticle

Lorini, R. ; Moretta, A. ; Valtorta, A. ; d'Annunzio, G. ; Cortona, L. ; Vitali, L. ; Bozzola, M. ; Severi, F. / Cytotoxic activity in children with insulin-dependent diabetes mellitus. In: Diabetes Research and Clinical Practice. 1994 ; Vol. 23, No. 1. pp. 37-42.
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