Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells

Stefania Meschini, Evelin Pellegrini, Maria Condello, Giovanni Occhionero, Sebastiano Delfine, Giancarlo Condello, Franco Mastrodonato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) ® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) ® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1%; SW480:11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86%; SW480:96%). Normal cells were not altered by (PsT + NAC) ® treatments.

Original languageEnglish
Article number1578
JournalMolecules
Volume22
Issue number9
DOIs
Publication statusPublished - Sep 20 2017

Fingerprint

Cells
Mitochondria
Flow cytometry
Poisons
Annexin A5
Cell death
Cell membranes
Vitamins
Pharmaceutical Preparations
Minerals
Assays
Buffers
Screening
Salts
Phosphates
Amino Acids

Keywords

  • Anticancer activity
  • Apoptosis
  • Mitochondrial dysfunction
  • Prunus spinosa
  • Trigno ecotype

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells. / Meschini, Stefania; Pellegrini, Evelin; Condello, Maria; Occhionero, Giovanni; Delfine, Sebastiano; Condello, Giancarlo; Mastrodonato, Franco.

In: Molecules, Vol. 22, No. 9, 1578, 20.09.2017.

Research output: Contribution to journalArticle

Meschini, Stefania ; Pellegrini, Evelin ; Condello, Maria ; Occhionero, Giovanni ; Delfine, Sebastiano ; Condello, Giancarlo ; Mastrodonato, Franco. / Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells. In: Molecules. 2017 ; Vol. 22, No. 9.
@article{1f17d21e6e2341f5bc0f96c2cbf17dfc,
title = "Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells",
abstract = "The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) {\circledR} has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) {\circledR} was about 40{\%} (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20{\%} depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1{\%}; SW480:11.6{\%}), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86{\%}; SW480:96{\%}). Normal cells were not altered by (PsT + NAC) {\circledR} treatments.",
keywords = "Anticancer activity, Apoptosis, Mitochondrial dysfunction, Prunus spinosa, Trigno ecotype",
author = "Stefania Meschini and Evelin Pellegrini and Maria Condello and Giovanni Occhionero and Sebastiano Delfine and Giancarlo Condello and Franco Mastrodonato",
year = "2017",
month = "9",
day = "20",
doi = "10.3390/molecules22091578",
language = "English",
volume = "22",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "9",

}

TY - JOUR

T1 - Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells

AU - Meschini, Stefania

AU - Pellegrini, Evelin

AU - Condello, Maria

AU - Occhionero, Giovanni

AU - Delfine, Sebastiano

AU - Condello, Giancarlo

AU - Mastrodonato, Franco

PY - 2017/9/20

Y1 - 2017/9/20

N2 - The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) ® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) ® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1%; SW480:11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86%; SW480:96%). Normal cells were not altered by (PsT + NAC) ® treatments.

AB - The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) ® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) ® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1%; SW480:11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86%; SW480:96%). Normal cells were not altered by (PsT + NAC) ® treatments.

KW - Anticancer activity

KW - Apoptosis

KW - Mitochondrial dysfunction

KW - Prunus spinosa

KW - Trigno ecotype

UR - http://www.scopus.com/inward/record.url?scp=85034962586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034962586&partnerID=8YFLogxK

U2 - 10.3390/molecules22091578

DO - 10.3390/molecules22091578

M3 - Article

AN - SCOPUS:85034962586

VL - 22

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 9

M1 - 1578

ER -