Abstract
The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) ® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) ® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1%; SW480:11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86%; SW480:96%). Normal cells were not altered by (PsT + NAC) ® treatments.
| Original language | English |
|---|---|
| Article number | 1578 |
| Journal | Molecules |
| Volume | 22 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 20 2017 |
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Keywords
- Anticancer activity
- Apoptosis
- Mitochondrial dysfunction
- Prunus spinosa
- Trigno ecotype
ASJC Scopus subject areas
- Organic Chemistry
Cite this
Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells. / Meschini, Stefania; Pellegrini, Evelin; Condello, Maria; Occhionero, Giovanni; Delfine, Sebastiano; Condello, Giancarlo; Mastrodonato, Franco.
In: Molecules, Vol. 22, No. 9, 1578, 20.09.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytotoxic and apoptotic activities of prunus spinosa trigno ecotype extract on human cancer cells
AU - Meschini, Stefania
AU - Pellegrini, Evelin
AU - Condello, Maria
AU - Occhionero, Giovanni
AU - Delfine, Sebastiano
AU - Condello, Giancarlo
AU - Mastrodonato, Franco
PY - 2017/9/20
Y1 - 2017/9/20
N2 - The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) ® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) ® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1%; SW480:11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86%; SW480:96%). Normal cells were not altered by (PsT + NAC) ® treatments.
AB - The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC) ® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC) ® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116:35.1%; SW480:11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116:86%; SW480:96%). Normal cells were not altered by (PsT + NAC) ® treatments.
KW - Anticancer activity
KW - Apoptosis
KW - Mitochondrial dysfunction
KW - Prunus spinosa
KW - Trigno ecotype
UR - http://www.scopus.com/inward/record.url?scp=85034962586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034962586&partnerID=8YFLogxK
U2 - 10.3390/molecules22091578
DO - 10.3390/molecules22091578
M3 - Article
AN - SCOPUS:85034962586
VL - 22
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 9
M1 - 1578
ER -