Cytotoxic and oxidative effects induced by man-made vitreous fibers (MMVFs) in a human mesothelial cell line

Delia Cavallo, Antonella Campopiano, Giorgia Cardinali, Stefano Casciardi, Paolo De Simone, Daniela Kovacs, Barbara Perniconi, Giuseppe Spagnoli, Cinzia L. Ursini, Carla Fanizza

Research output: Contribution to journalArticle

Abstract

The introduction of man-made vitreous fibers (MMVFs) as a substitute for asbestos in industrial and residential applications raises concerns about their potential health hazards. The aim of our study was to assess cytotoxic and oxidative effects induced on a human mesothelial cell line (MeT-5A) by exposure to glass wool (GW), rock wool (RW) and refractory ceramic fibers (RCF) in comparison with crocidolite asbestos (CR). MeT-5A cells were exposed for 24 h to 2, 5 and 10 μg/cm2 of MMVF and crocidolite fibers and analysed by scanning electron microscope (SEM) for cell surface alterations. Cells were exposed for 2 h to 1, 2, 5 and 10 μg/cm2 of the same fibers and analysed by enzyme Fpg-modified comet test for direct and oxidative DNA damage. SEM revealed loss of microvilli in cells exposed to RCF and numerous blebs in cells exposed to higher doses of RW. Comet test showed significant direct DNA damage in cells exposed to RCF even at the lowest dose. Comet test with Fpg, that permits the detection of oxided DNA bases, showed significant oxidative DNA damage in cells exposed to higher doses of RW. The presence of DNA damage and alterations of cell surface induced by low doses of RCF and the presence of oxidative DNA damage and blebs on cell surface in cells exposed to higher dose of RW suggest possible cytotoxic, oxidative and genotoxic effects for these MMVFs.

Original languageEnglish
Pages (from-to)219-229
Number of pages11
JournalToxicology
Volume201
Issue number1-3
DOIs
Publication statusPublished - Sep 1 2004

Keywords

  • Comet assay
  • Cytotoxic effects
  • Human mesothelial cells
  • Man-made vitreous fibers
  • Oxidative damage
  • SEM

ASJC Scopus subject areas

  • Toxicology

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    Cavallo, D., Campopiano, A., Cardinali, G., Casciardi, S., Simone, P. D., Kovacs, D., Perniconi, B., Spagnoli, G., Ursini, C. L., & Fanizza, C. (2004). Cytotoxic and oxidative effects induced by man-made vitreous fibers (MMVFs) in a human mesothelial cell line. Toxicology, 201(1-3), 219-229. https://doi.org/10.1016/j.tox.2004.04.017