Cytotoxicity of human peripheral blood monocytes against Trichomonas vaginalis

A. Mantovani, N. Polentarutti, G. Peri, G. Martinotti, S. Landolfo

Research output: Contribution to journalArticle

Abstract

Human peripheral blood monocytes were purified by adherence on microexudate-coated plastic and detached by exposure to 1 mM ethylenediamine tetracetic acid. Lysis of Trichomonas vaginalis was measured as release of 3H-thymidine from prelablelled protozoa after 48 hr of incubation. Human blood monocytes had appreciable levels of spontaneous cytotoxicity against Trichomonas vaginalis at effector-to-target cell ratios ranging from 3 : 1 to 25 : 1. Cytotoxicity was expressed with fetal bovine serum and human AB serum. Unseparated mononuclear cells, containing 10-25% monocytes as assessed by staining with non-specific esterases, were significantly cytotoxic against Trichomonas vaginalis but cytolysis levels were lower than those of purified monocytes. Lymphoid cells depleted of adherent cells by repeated incubations on plastic (less than 2% esterase-positive cells) had little cytotoxicity against these protozoa. In vitro exposure to silica caused 40-75% inhibition of the cytotoxic capacity of monocyte preparations. The natural cytotoxic capacity of human monocytes against Trichomonas vaginalis could represent one line of resistance against these protozoa.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalClinical and Experimental Immunology
Volume46
Issue number2
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Immunology

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