TY - JOUR
T1 - Cytotoxicity on tumor cells of peripheral blood monocytes and tumor-associated macrophages in patients with ascites ovarian tumors
AU - Mantovani, Alberto
AU - Polentarutti, Nadia
AU - Peri, Giuseppe
AU - Shavit, Zvi Bar
AU - Vecchi, Annunciata
PY - 1980
Y1 - 1980
N2 - Mononuclear phagocytes were Isolated from the peripheral blood (PB) and ascites tumors of 35 patients with epithelial ovarian tumors. After 48 hours of Incubation with the TU5 tumor, tumor-associated macrophages (TAM) and PB monocytes from cancer patients showed lower cytolytic activity than did control cells, but by 72 hours there was little difference between control and ovarian cancer effector cells. Primary ovarian carcinoma cultures were heterogeneous In their susceptibility to macrophage cytotoxicity. Tumor cells from 7 patients were significantly lysed by monocytes and macrophages, whereas four ovarian cancer cell preparations were resistant to cytotoxicity. A “feeding” effect of mononuclear phagocytes on non- lysable tumor cells was detected In terms of both lower [3H]thymidlne-release values In the cytolysis assay and Increased proliferation in cytostasis assays. Thus patients with ovarian carcinomatous ascites PB monocytes and TAM had impaired cytotoxicity against a tumor cell line, and primary ovarian carcinoma cultures were heterogeneous In their Interaction with mononuclear phagocytes.
AB - Mononuclear phagocytes were Isolated from the peripheral blood (PB) and ascites tumors of 35 patients with epithelial ovarian tumors. After 48 hours of Incubation with the TU5 tumor, tumor-associated macrophages (TAM) and PB monocytes from cancer patients showed lower cytolytic activity than did control cells, but by 72 hours there was little difference between control and ovarian cancer effector cells. Primary ovarian carcinoma cultures were heterogeneous In their susceptibility to macrophage cytotoxicity. Tumor cells from 7 patients were significantly lysed by monocytes and macrophages, whereas four ovarian cancer cell preparations were resistant to cytotoxicity. A “feeding” effect of mononuclear phagocytes on non- lysable tumor cells was detected In terms of both lower [3H]thymidlne-release values In the cytolysis assay and Increased proliferation in cytostasis assays. Thus patients with ovarian carcinomatous ascites PB monocytes and TAM had impaired cytotoxicity against a tumor cell line, and primary ovarian carcinoma cultures were heterogeneous In their Interaction with mononuclear phagocytes.
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U2 - 10.1093/jnci/64.6.1307
DO - 10.1093/jnci/64.6.1307
M3 - Article
C2 - 6246299
AN - SCOPUS:0018857471
VL - 64
SP - 1307
EP - 1315
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 6
ER -