Cytotoxicity testing of cyanoacrylates using direct contact assay on cell cultures

Gabriela Ciapetti, Susanna Stea, Elisabetta Cenni, Alessandra Sudanese, Daniela Marraro, Aldo Toni, Arturo Pizzoferrato

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The use of a tissue adhesive for surgical procedures has prompted a large number of clinical and experimental studies. Alkyl-2-cyanoacrylate esters constitute a family of adhesives with good mechanical properties but their biological compatibility has to be assessed. In this study the cytotoxicity of three commercially available cyanoacrylates and one of unknown composition has been determined. The first part of the study deals with direct contact testing procedures using L 929 cells challenged with drops of adhesives: cell morphology, cell growth and bacterial growth inhibition were assayed. Testing methods included cell viability assay using vital dyes, cell growth measurement using crystal violet staining uptake and bacterial growth assay using S. aureus growth inhibition. All the cyanoacrylate adhesives tested were found to be cytotoxic and to inhibit cell proliferation: differences between the cyanoacrylates were found.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalBiomaterials
Volume15
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Cyanoacrylates
Cytotoxicity
Cell culture
Assays
Adhesives
Cell Culture Techniques
Cell growth
Testing
Growth
Tissue Adhesives
Gentian Violet
Cell proliferation
Esters
Coloring Agents
Dyes
Cells
Tissue
Cell Survival
Mechanical properties
Crystals

Keywords

  • cell culture
  • Cyanoacrylate
  • cytotoxicity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Cytotoxicity testing of cyanoacrylates using direct contact assay on cell cultures. / Ciapetti, Gabriela; Stea, Susanna; Cenni, Elisabetta; Sudanese, Alessandra; Marraro, Daniela; Toni, Aldo; Pizzoferrato, Arturo.

In: Biomaterials, Vol. 15, No. 1, 1994, p. 63-67.

Research output: Contribution to journalArticle

Ciapetti, Gabriela ; Stea, Susanna ; Cenni, Elisabetta ; Sudanese, Alessandra ; Marraro, Daniela ; Toni, Aldo ; Pizzoferrato, Arturo. / Cytotoxicity testing of cyanoacrylates using direct contact assay on cell cultures. In: Biomaterials. 1994 ; Vol. 15, No. 1. pp. 63-67.
@article{f5fd45c05d4d403e9f4870b6142fb692,
title = "Cytotoxicity testing of cyanoacrylates using direct contact assay on cell cultures",
abstract = "The use of a tissue adhesive for surgical procedures has prompted a large number of clinical and experimental studies. Alkyl-2-cyanoacrylate esters constitute a family of adhesives with good mechanical properties but their biological compatibility has to be assessed. In this study the cytotoxicity of three commercially available cyanoacrylates and one of unknown composition has been determined. The first part of the study deals with direct contact testing procedures using L 929 cells challenged with drops of adhesives: cell morphology, cell growth and bacterial growth inhibition were assayed. Testing methods included cell viability assay using vital dyes, cell growth measurement using crystal violet staining uptake and bacterial growth assay using S. aureus growth inhibition. All the cyanoacrylate adhesives tested were found to be cytotoxic and to inhibit cell proliferation: differences between the cyanoacrylates were found.",
keywords = "cell culture, Cyanoacrylate, cytotoxicity",
author = "Gabriela Ciapetti and Susanna Stea and Elisabetta Cenni and Alessandra Sudanese and Daniela Marraro and Aldo Toni and Arturo Pizzoferrato",
year = "1994",
doi = "10.1016/0142-9612(94)90199-6",
language = "English",
volume = "15",
pages = "63--67",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Cytotoxicity testing of cyanoacrylates using direct contact assay on cell cultures

AU - Ciapetti, Gabriela

AU - Stea, Susanna

AU - Cenni, Elisabetta

AU - Sudanese, Alessandra

AU - Marraro, Daniela

AU - Toni, Aldo

AU - Pizzoferrato, Arturo

PY - 1994

Y1 - 1994

N2 - The use of a tissue adhesive for surgical procedures has prompted a large number of clinical and experimental studies. Alkyl-2-cyanoacrylate esters constitute a family of adhesives with good mechanical properties but their biological compatibility has to be assessed. In this study the cytotoxicity of three commercially available cyanoacrylates and one of unknown composition has been determined. The first part of the study deals with direct contact testing procedures using L 929 cells challenged with drops of adhesives: cell morphology, cell growth and bacterial growth inhibition were assayed. Testing methods included cell viability assay using vital dyes, cell growth measurement using crystal violet staining uptake and bacterial growth assay using S. aureus growth inhibition. All the cyanoacrylate adhesives tested were found to be cytotoxic and to inhibit cell proliferation: differences between the cyanoacrylates were found.

AB - The use of a tissue adhesive for surgical procedures has prompted a large number of clinical and experimental studies. Alkyl-2-cyanoacrylate esters constitute a family of adhesives with good mechanical properties but their biological compatibility has to be assessed. In this study the cytotoxicity of three commercially available cyanoacrylates and one of unknown composition has been determined. The first part of the study deals with direct contact testing procedures using L 929 cells challenged with drops of adhesives: cell morphology, cell growth and bacterial growth inhibition were assayed. Testing methods included cell viability assay using vital dyes, cell growth measurement using crystal violet staining uptake and bacterial growth assay using S. aureus growth inhibition. All the cyanoacrylate adhesives tested were found to be cytotoxic and to inhibit cell proliferation: differences between the cyanoacrylates were found.

KW - cell culture

KW - Cyanoacrylate

KW - cytotoxicity

UR - http://www.scopus.com/inward/record.url?scp=0027953293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027953293&partnerID=8YFLogxK

U2 - 10.1016/0142-9612(94)90199-6

DO - 10.1016/0142-9612(94)90199-6

M3 - Article

C2 - 8161661

AN - SCOPUS:0027953293

VL - 15

SP - 63

EP - 67

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 1

ER -