Cytotoxicity testing of materials with limited in vivo exposure is affected by the duration of cell-material contact

Gabriela Ciapetti, Donatella Granchi, Susanna Stea, Lucia Savarino, Elisabetta Verri, Alessandra Gori, Franca Savioli, Lucio Montanaro

Research output: Contribution to journalArticle

Abstract

Silicones for dental impression largely are used to record the geometry of hard and soft dental tissues. They are considered to be medical devices, and the assessment of cytotoxicity is a necessary step in the evaluation of their biocompatibility. Extracts of six addition-type and six condensation- type silicones have been tested with L929 cells according to the ISO 10993 - Part 5 standard. The cytotoxicity was evaluated by three different methods: neutral red uptake, propidium iodide (PI) staining, and amido black staining. According to the selected specific assay, contact between cells and material extracts was maintained for 24 h in the first series of experiments; then, considering that in vivo application of these materials is restricted to a few minutes, additional experiments were performed after 1 h of cell/extract contact. Analysis of the results showed that the addition-type silicones are nontoxic even when tested after prolonged exposure of the cells to the materials while the condensation-type silicones were cytotoxic at 24 h of incubation. Nevertheless, harm to the patient actually could be negligible, considering its very short time of exposure in vivo. This is supported by our finding that most are not toxic after 1 h. We suggest that the experimental conditions of cytotoxicity testing have to be relevant to the in vivo situation; accordingly, the time of exposure should be designed carefully.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalJournal of Biomedical Materials Research
Volume42
Issue number4
DOIs
Publication statusPublished - Dec 15 1998

Fingerprint

Materials Testing
Silicones
Cytotoxicity
Testing
Cell Extracts
Condensation
Amido Black
Tooth
Staining and Labeling
Patient Harm
Neutral Red
Propidium
Poisons
Biocompatibility
Assays
Experiments
Tissue
Equipment and Supplies
Geometry

Keywords

  • Additiontype-silicones
  • Condensation- type-silicones
  • Cytotoxicity
  • Impression materials

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Cytotoxicity testing of materials with limited in vivo exposure is affected by the duration of cell-material contact. / Ciapetti, Gabriela; Granchi, Donatella; Stea, Susanna; Savarino, Lucia; Verri, Elisabetta; Gori, Alessandra; Savioli, Franca; Montanaro, Lucio.

In: Journal of Biomedical Materials Research, Vol. 42, No. 4, 15.12.1998, p. 485-490.

Research output: Contribution to journalArticle

Ciapetti, Gabriela ; Granchi, Donatella ; Stea, Susanna ; Savarino, Lucia ; Verri, Elisabetta ; Gori, Alessandra ; Savioli, Franca ; Montanaro, Lucio. / Cytotoxicity testing of materials with limited in vivo exposure is affected by the duration of cell-material contact. In: Journal of Biomedical Materials Research. 1998 ; Vol. 42, No. 4. pp. 485-490.
@article{28c3bb86aeb04f03b734d64a9a781f2b,
title = "Cytotoxicity testing of materials with limited in vivo exposure is affected by the duration of cell-material contact",
abstract = "Silicones for dental impression largely are used to record the geometry of hard and soft dental tissues. They are considered to be medical devices, and the assessment of cytotoxicity is a necessary step in the evaluation of their biocompatibility. Extracts of six addition-type and six condensation- type silicones have been tested with L929 cells according to the ISO 10993 - Part 5 standard. The cytotoxicity was evaluated by three different methods: neutral red uptake, propidium iodide (PI) staining, and amido black staining. According to the selected specific assay, contact between cells and material extracts was maintained for 24 h in the first series of experiments; then, considering that in vivo application of these materials is restricted to a few minutes, additional experiments were performed after 1 h of cell/extract contact. Analysis of the results showed that the addition-type silicones are nontoxic even when tested after prolonged exposure of the cells to the materials while the condensation-type silicones were cytotoxic at 24 h of incubation. Nevertheless, harm to the patient actually could be negligible, considering its very short time of exposure in vivo. This is supported by our finding that most are not toxic after 1 h. We suggest that the experimental conditions of cytotoxicity testing have to be relevant to the in vivo situation; accordingly, the time of exposure should be designed carefully.",
keywords = "Additiontype-silicones, Condensation- type-silicones, Cytotoxicity, Impression materials",
author = "Gabriela Ciapetti and Donatella Granchi and Susanna Stea and Lucia Savarino and Elisabetta Verri and Alessandra Gori and Franca Savioli and Lucio Montanaro",
year = "1998",
month = "12",
day = "15",
doi = "10.1002/(SICI)1097-4636(19981215)42:4<485::AID-JBM2>3.0.CO;2-7",
language = "English",
volume = "42",
pages = "485--490",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Cytotoxicity testing of materials with limited in vivo exposure is affected by the duration of cell-material contact

AU - Ciapetti, Gabriela

AU - Granchi, Donatella

AU - Stea, Susanna

AU - Savarino, Lucia

AU - Verri, Elisabetta

AU - Gori, Alessandra

AU - Savioli, Franca

AU - Montanaro, Lucio

PY - 1998/12/15

Y1 - 1998/12/15

N2 - Silicones for dental impression largely are used to record the geometry of hard and soft dental tissues. They are considered to be medical devices, and the assessment of cytotoxicity is a necessary step in the evaluation of their biocompatibility. Extracts of six addition-type and six condensation- type silicones have been tested with L929 cells according to the ISO 10993 - Part 5 standard. The cytotoxicity was evaluated by three different methods: neutral red uptake, propidium iodide (PI) staining, and amido black staining. According to the selected specific assay, contact between cells and material extracts was maintained for 24 h in the first series of experiments; then, considering that in vivo application of these materials is restricted to a few minutes, additional experiments were performed after 1 h of cell/extract contact. Analysis of the results showed that the addition-type silicones are nontoxic even when tested after prolonged exposure of the cells to the materials while the condensation-type silicones were cytotoxic at 24 h of incubation. Nevertheless, harm to the patient actually could be negligible, considering its very short time of exposure in vivo. This is supported by our finding that most are not toxic after 1 h. We suggest that the experimental conditions of cytotoxicity testing have to be relevant to the in vivo situation; accordingly, the time of exposure should be designed carefully.

AB - Silicones for dental impression largely are used to record the geometry of hard and soft dental tissues. They are considered to be medical devices, and the assessment of cytotoxicity is a necessary step in the evaluation of their biocompatibility. Extracts of six addition-type and six condensation- type silicones have been tested with L929 cells according to the ISO 10993 - Part 5 standard. The cytotoxicity was evaluated by three different methods: neutral red uptake, propidium iodide (PI) staining, and amido black staining. According to the selected specific assay, contact between cells and material extracts was maintained for 24 h in the first series of experiments; then, considering that in vivo application of these materials is restricted to a few minutes, additional experiments were performed after 1 h of cell/extract contact. Analysis of the results showed that the addition-type silicones are nontoxic even when tested after prolonged exposure of the cells to the materials while the condensation-type silicones were cytotoxic at 24 h of incubation. Nevertheless, harm to the patient actually could be negligible, considering its very short time of exposure in vivo. This is supported by our finding that most are not toxic after 1 h. We suggest that the experimental conditions of cytotoxicity testing have to be relevant to the in vivo situation; accordingly, the time of exposure should be designed carefully.

KW - Additiontype-silicones

KW - Condensation- type-silicones

KW - Cytotoxicity

KW - Impression materials

UR - http://www.scopus.com/inward/record.url?scp=0032534526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032534526&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4636(19981215)42:4<485::AID-JBM2>3.0.CO;2-7

DO - 10.1002/(SICI)1097-4636(19981215)42:4<485::AID-JBM2>3.0.CO;2-7

M3 - Article

C2 - 9827670

AN - SCOPUS:0032534526

VL - 42

SP - 485

EP - 490

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 4

ER -