D-Glucose does not catabolite repress a transketolase-deficient D-ribose-producing Bacillus subtilis mutant strain

P. De Wulf, W. Soetaert, D. Schwengers, E. J. Vandamme

Research output: Contribution to journalArticle

Abstract

When Bacillus subtilis strain ATCC 21951, a transketolase-deficient D-ribose-producing mutant, was grown on D-glucose plus a second substrate which is metabolized via the oxidative pentose phosphate cycle (D-gluconic acid, D-xylose, L-arabinose or D-xylitol), D-glucose did not catabolite repress metabolism of the second carbon source. The D-ribose yield obtained with the simultaneously converted carbon substrates, significantly exceeded that when only D-glucose was used. In addition, the concentration of glycolytic by-products and the fermentation time significantly decreased. Based on these findings, a fermentation process was developed with B. subtilis strain ATCC 21951 in which D-glucose (100 g L -1) and D-gluconic acid (50 g L -1) were converted into 45 g L -1 of D-ribose and 7.5 g L -1 of acetoin, A second process, based on D-glucose and D-xylose (100 g L -1 each), yielded 60 g L -1 of D-ribose and 4 g L -1 of acetoin plus 2,3-butanediol. Both mixed carbon source fermentations provide excellent alternatives to the less efficient D-glucose-based processes used so far.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalJournal of Industrial Microbiology
Volume17
Issue number2
Publication statusPublished - 1996

Keywords

  • Bacillus subtilis
  • Catabolite repression
  • D-Ribose
  • Pentose phosphate cycle
  • Transketolase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Bioengineering
  • Microbiology

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