D-ribose and deoxy-d-ribose induce apoptosis in human quiescent peripheral blood mononuclear cells

D. Barbieri, E. Grassilli, D. Monti, S. Salvioli, M. G. Franceschini, A. Franchini, E. Bellesia, P. Salomoni, P. Negro, M. Capri, L. Troiano, A. Cossarizza, C. Franceschi

Research output: Contribution to journalArticle

Abstract

In previous papers we reported that D(-)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(-)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inhibited by N- acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(-)-ribose and 2- deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes.

Original languageEnglish
Pages (from-to)1109-1116
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number3
DOIs
Publication statusPublished - 1994

Fingerprint

Ribose
Blood Cells
Blood
Apoptosis
Sugars
Cells
Oxidative stress
Lymphocytes
Flow cytometry
Agar Gel Electrophoresis
Acetylcysteine
DNA Fragmentation
Electrophoresis
Metabolism
Sepharose
Glutathione
Flow Cytometry
Animals
Oxidative Stress
Gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

D-ribose and deoxy-d-ribose induce apoptosis in human quiescent peripheral blood mononuclear cells. / Barbieri, D.; Grassilli, E.; Monti, D.; Salvioli, S.; Franceschini, M. G.; Franchini, A.; Bellesia, E.; Salomoni, P.; Negro, P.; Capri, M.; Troiano, L.; Cossarizza, A.; Franceschi, C.

In: Biochemical and Biophysical Research Communications, Vol. 201, No. 3, 1994, p. 1109-1116.

Research output: Contribution to journalArticle

Barbieri, D, Grassilli, E, Monti, D, Salvioli, S, Franceschini, MG, Franchini, A, Bellesia, E, Salomoni, P, Negro, P, Capri, M, Troiano, L, Cossarizza, A & Franceschi, C 1994, 'D-ribose and deoxy-d-ribose induce apoptosis in human quiescent peripheral blood mononuclear cells', Biochemical and Biophysical Research Communications, vol. 201, no. 3, pp. 1109-1116. https://doi.org/10.1006/bbrc.1994.1820
Barbieri, D. ; Grassilli, E. ; Monti, D. ; Salvioli, S. ; Franceschini, M. G. ; Franchini, A. ; Bellesia, E. ; Salomoni, P. ; Negro, P. ; Capri, M. ; Troiano, L. ; Cossarizza, A. ; Franceschi, C. / D-ribose and deoxy-d-ribose induce apoptosis in human quiescent peripheral blood mononuclear cells. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 201, No. 3. pp. 1109-1116.
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