TY - JOUR
T1 - D-ribose and deoxy-d-ribose induce apoptosis in human quiescent peripheral blood mononuclear cells
AU - Barbieri, D.
AU - Grassilli, E.
AU - Monti, D.
AU - Salvioli, S.
AU - Franceschini, M. G.
AU - Franchini, A.
AU - Bellesia, E.
AU - Salomoni, P.
AU - Negro, P.
AU - Capri, M.
AU - Troiano, L.
AU - Cossarizza, A.
AU - Franceschi, C.
PY - 1994
Y1 - 1994
N2 - In previous papers we reported that D(-)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(-)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inhibited by N- acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(-)-ribose and 2- deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes.
AB - In previous papers we reported that D(-)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(-)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inhibited by N- acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(-)-ribose and 2- deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes.
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U2 - 10.1006/bbrc.1994.1820
DO - 10.1006/bbrc.1994.1820
M3 - Article
C2 - 8024552
AN - SCOPUS:0028168743
VL - 201
SP - 1109
EP - 1116
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -