TY - JOUR
T1 - D-ribose inhibits DNA repair synthesis in human lymphocytes
AU - Zunica, G.
AU - Marini, M.
AU - Brunelli, M. A.
AU - Chiricolo, M.
AU - Franceschi, C.
PY - 1986/7/31
Y1 - 1986/7/31
N2 - D-ribose is cytotoxic for quiescent human lymphocytes and severely inhibits their PHA-induced proliferation at concentrations (25-50 mM) at which other simple sugars are ineffective. In order to explain these effects, DNA repair synthesis was evaluated in PHA-stimulated human lymphocytes treated with hydroxyurea and irradiated. D-ribose, in contrast to other reducing sugars, did not induce repair synthesis and therefore did not apparently damage DNA in a direct way, although it markedly inhibited gamma ray-induced repair. Taking into account that lymphocytes must rejoin physiologically-formed DNA strand breaks in order to enter the cell cycle, we suggest that D-ribose exerts its cytotoxic activity by interfering with metabolic pathways critical for the repair of DNA breaks.
AB - D-ribose is cytotoxic for quiescent human lymphocytes and severely inhibits their PHA-induced proliferation at concentrations (25-50 mM) at which other simple sugars are ineffective. In order to explain these effects, DNA repair synthesis was evaluated in PHA-stimulated human lymphocytes treated with hydroxyurea and irradiated. D-ribose, in contrast to other reducing sugars, did not induce repair synthesis and therefore did not apparently damage DNA in a direct way, although it markedly inhibited gamma ray-induced repair. Taking into account that lymphocytes must rejoin physiologically-formed DNA strand breaks in order to enter the cell cycle, we suggest that D-ribose exerts its cytotoxic activity by interfering with metabolic pathways critical for the repair of DNA breaks.
UR - http://www.scopus.com/inward/record.url?scp=0022522783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022522783&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(86)80549-6
DO - 10.1016/S0006-291X(86)80549-6
M3 - Article
C2 - 3091020
AN - SCOPUS:0022522783
VL - 138
SP - 673
EP - 678
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -