D-ribose inhibits DNA repair synthesis in human lymphocytes

G. Zunica, M. Marini, M. A. Brunelli, M. Chiricolo, C. Franceschi

Research output: Contribution to journalArticle

Abstract

D-ribose is cytotoxic for quiescent human lymphocytes and severely inhibits their PHA-induced proliferation at concentrations (25-50 mM) at which other simple sugars are ineffective. In order to explain these effects, DNA repair synthesis was evaluated in PHA-stimulated human lymphocytes treated with hydroxyurea and irradiated. D-ribose, in contrast to other reducing sugars, did not induce repair synthesis and therefore did not apparently damage DNA in a direct way, although it markedly inhibited gamma ray-induced repair. Taking into account that lymphocytes must rejoin physiologically-formed DNA strand breaks in order to enter the cell cycle, we suggest that D-ribose exerts its cytotoxic activity by interfering with metabolic pathways critical for the repair of DNA breaks.

Original languageEnglish
Pages (from-to)673-678
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number2
DOIs
Publication statusPublished - Jul 31 1986

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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