DADLE induces a reversible hibernation-like state in HeLa cells

Lorella Vecchio, Cristiana Soldani, Maria Grazia Bottone, Manuela Malatesta, Terence E. Martin, Lawrence I. Rothblum, Carlo Pellicciari, Marco Biggiogera

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

[D-Ala(2)-D-Leu(5)-Enkephalin] (DADLE) can induce hibernation when injected into ground squirrels in summer and is able to increase the survival time of explanted organs such as liver and lung. Since cell metabolism is a target of this peptide, we have treated HeLa cells with DADLE and investigated its possible effect on transcription and proliferation as well as the resumption of metabolic activity after treatment. The labelling for Pol I, Pol II and for splicing factors such as snRNPs and SC-35 decreased after treatment as did the nucleolar labelling for UBF. In treated cells, several spherical nuclear bodies were found to be labelled for hnRNPs. In parallel, the number of proliferating cells decreased after treatment with DADLE. After recovery, there was a gradual resumption of cell function: transcription and splicing factors had a distribution similar to that of controls; proliferation resumed; nuclear bodies, representing storage sites for RNPs, disappeared.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalHistochemistry and Cell Biology
Volume125
Issue number1-2
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Leucine-2-Alanine Enkephalin
hibernation
Hibernation
Transcription
HeLa Cells
Labeling
splicing
cells
Metabolism
Liver
Peptides
marking
ground squirrels
Sciuridae
Cells
Recovery
metabolism
Transcription Factors
Cell Count
liver

Keywords

  • Electron microscopy
  • Induced hypometabolic state
  • Nucleus
  • Proliferation
  • Transcription

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Vecchio, L., Soldani, C., Bottone, M. G., Malatesta, M., Martin, T. E., Rothblum, L. I., ... Biggiogera, M. (2006). DADLE induces a reversible hibernation-like state in HeLa cells. Histochemistry and Cell Biology, 125(1-2), 193-201. https://doi.org/10.1007/s00418-005-0085-x

DADLE induces a reversible hibernation-like state in HeLa cells. / Vecchio, Lorella; Soldani, Cristiana; Bottone, Maria Grazia; Malatesta, Manuela; Martin, Terence E.; Rothblum, Lawrence I.; Pellicciari, Carlo; Biggiogera, Marco.

In: Histochemistry and Cell Biology, Vol. 125, No. 1-2, 01.2006, p. 193-201.

Research output: Contribution to journalArticle

Vecchio, L, Soldani, C, Bottone, MG, Malatesta, M, Martin, TE, Rothblum, LI, Pellicciari, C & Biggiogera, M 2006, 'DADLE induces a reversible hibernation-like state in HeLa cells', Histochemistry and Cell Biology, vol. 125, no. 1-2, pp. 193-201. https://doi.org/10.1007/s00418-005-0085-x
Vecchio, Lorella ; Soldani, Cristiana ; Bottone, Maria Grazia ; Malatesta, Manuela ; Martin, Terence E. ; Rothblum, Lawrence I. ; Pellicciari, Carlo ; Biggiogera, Marco. / DADLE induces a reversible hibernation-like state in HeLa cells. In: Histochemistry and Cell Biology. 2006 ; Vol. 125, No. 1-2. pp. 193-201.
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