Abstract
We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. "Cara Cara" and cv. "Bahia" on faecal microbiota and metabolome using an integrated meta-omics approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on 1H NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group.
| Original language | English |
|---|---|
| Pages (from-to) | 1381-1391 |
| Number of pages | 11 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 67 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Feb 6 2019 |
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Keywords
- Adult
- Bacteria/classification
- Brazil
- Citrus sinensis/chemistry
- Female
- Fruit/chemistry
- Fruit and Vegetable Juices/analysis
- Gastrointestinal Microbiome
- Gastrointestinal Tract/metabolism
- Humans
- Male
- Middle Aged
- Young Adult
Cite this
Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omics Approach. / Brasili, Elisa; Hassimotto, Neuza Mariko Aymoto; Del Chierico, Federica; Marini, Federico; Quagliariello, Andrea; Sciubba, Fabio; Miccheli, Alfredo; Putignani, Lorenza; Lajolo, Franco.
In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 5, 06.02.2019, p. 1381-1391.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omics Approach
AU - Brasili, Elisa
AU - Hassimotto, Neuza Mariko Aymoto
AU - Del Chierico, Federica
AU - Marini, Federico
AU - Quagliariello, Andrea
AU - Sciubba, Fabio
AU - Miccheli, Alfredo
AU - Putignani, Lorenza
AU - Lajolo, Franco
PY - 2019/2/6
Y1 - 2019/2/6
N2 - We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. "Cara Cara" and cv. "Bahia" on faecal microbiota and metabolome using an integrated meta-omics approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on 1H NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group.
AB - We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. "Cara Cara" and cv. "Bahia" on faecal microbiota and metabolome using an integrated meta-omics approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on 1H NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group.
KW - Adult
KW - Bacteria/classification
KW - Brazil
KW - Citrus sinensis/chemistry
KW - Female
KW - Fruit/chemistry
KW - Fruit and Vegetable Juices/analysis
KW - Gastrointestinal Microbiome
KW - Gastrointestinal Tract/metabolism
KW - Humans
KW - Male
KW - Middle Aged
KW - Young Adult
U2 - 10.1021/acs.jafc.8b05408
DO - 10.1021/acs.jafc.8b05408
M3 - Article
C2 - 30644740
VL - 67
SP - 1381
EP - 1391
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 5
ER -