Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omics Approach

Elisa Brasili, Neuza Mariko Aymoto Hassimotto, Federica Del Chierico, Federico Marini, Andrea Quagliariello, Fabio Sciubba, Alfredo Miccheli, Lorenza Putignani, Franco Lajolo

Research output: Contribution to journalArticle

Abstract

We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. "Cara Cara" and cv. "Bahia" on faecal microbiota and metabolome using an integrated meta-omics approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on 1H NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group.

Original languageEnglish
Pages (from-to)1381-1391
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume67
Issue number5
DOIs
Publication statusPublished - Feb 6 2019

Fingerprint

formic acid
orange juice
Citrus sinensis
Metabolites
intestinal microorganisms
Urocanic Acid
Clostridium
juices
Microbiota
Inositol
Choline
Veillonellaceae
metabolites
Nuclear magnetic resonance spectroscopy
Lysine
Arginine
metabolome
formates
metabolomics
choline

Keywords

  • Adult
  • Bacteria/classification
  • Brazil
  • Citrus sinensis/chemistry
  • Female
  • Fruit/chemistry
  • Fruit and Vegetable Juices/analysis
  • Gastrointestinal Microbiome
  • Gastrointestinal Tract/metabolism
  • Humans
  • Male
  • Middle Aged
  • Young Adult

Cite this

Daily Consumption of Orange Juice from Citrus sinensis L. Osbeck cv. Cara Cara and cv. Bahia Differently Affects Gut Microbiota Profiling as Unveiled by an Integrated Meta-Omics Approach. / Brasili, Elisa; Hassimotto, Neuza Mariko Aymoto; Del Chierico, Federica; Marini, Federico; Quagliariello, Andrea; Sciubba, Fabio; Miccheli, Alfredo; Putignani, Lorenza; Lajolo, Franco.

In: Journal of Agricultural and Food Chemistry, Vol. 67, No. 5, 06.02.2019, p. 1381-1391.

Research output: Contribution to journalArticle

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AU - Hassimotto, Neuza Mariko Aymoto

AU - Del Chierico, Federica

AU - Marini, Federico

AU - Quagliariello, Andrea

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