De novo a-to-i rna editing discovery in lncrna

Domenico Alessandro Silvestris, Chiara Scopa, Sara Hanchi, Franco Locatelli, Angela Gallo

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Adenosine to inosine (A-to-I) RNA editing is the most frequent editing event in humans. It converts adenosine to inosine in double-stranded RNA regions (in coding and noncoding RNAs) through the action of the adenosine deaminase acting on RNA (ADAR) enzymes. Long non-coding RNAs, particularly abundant in the brain, account for a large fraction of the human transcriptome, and their important regulatory role is becoming progressively evident in both normal and transformed cells. Results: Herein, we present a bioinformatic analysis to generate a comprehensive inosinome picture in long non-coding RNAs (lncRNAs), using an ad hoc index and searching for de novo editing events in the normal brain cortex as well as in glioblastoma, a highly aggressive human brain cancer. We discovered >10,000 new sites and 335 novel lncRNAs that undergo editing, never reported before. We found a generalized downregulation of editing at multiple lncRNA sites in glioblastoma samples when compared to the normal brain cortex. Conclusion: Overall, our study discloses a novel layer of complexity that controls lncRNAs in the brain and brain cancer.

Original languageEnglish
Article number2959
Pages (from-to)1-13
Number of pages13
JournalCancers
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 2020

Keywords

  • Brain
  • Glioblastoma
  • LncRNA
  • RNA editing

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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