De Novo Missense Variants in FBXW11 Cause Diverse Developmental Phenotypes Including Brain, Eye, and Digit Anomalies

Richard J. Holt, Rodrigo M. Young, Berta Crespo, Fabiola Ceroni, Cynthia J. Curry, Emanuele Bellacchio, Dorine A. Bax, Andrea Ciolfi, Marleen Simon, Christina R. Fagerberg, Ellen van Binsbergen, Alessandro De Luca, Luigi Memo, William B. Dobyns, Alaa Afif Mohammed, Samuel J.H. Clokie, Celia Zazo Seco, Yong Hui Jiang, Kristina P. Sørensen, Helle AndersenJennifer Sullivan, Zöe Powis, Anna Chassevent, Constance Smith-Hicks, Slavé Petrovski, Thalia Antoniadi, Vandana Shashi, Bruce D. Gelb, Stephen W. Wilson, Dianne Gerrelli, Marco Tartaglia, Nicolas Chassaing, Patrick Calvas, Nicola K. Ragge

Research output: Contribution to journalArticle

Abstract

The identification of genetic variants implicated in human developmental disorders has been revolutionized by second-generation sequencing combined with international pooling of cases. Here, we describe seven individuals who have diverse yet overlapping developmental anomalies, and who all have de novo missense FBXW11 variants identified by whole exome or whole genome sequencing and not reported in the gnomAD database. Their phenotypes include striking neurodevelopmental, digital, jaw, and eye anomalies, and in one individual, features resembling Noonan syndrome, a condition caused by dysregulated RAS signaling. FBXW11 encodes an F-box protein, part of the Skp1-cullin-F-box (SCF) ubiquitin ligase complex, involved in ubiquitination and proteasomal degradation and thus fundamental to many protein regulatory processes. FBXW11 targets include β-catenin and GLI transcription factors, key mediators of Wnt and Hh signaling, respectively, critical to digital, neurological, and eye development. Structural analyses indicate affected residues cluster at the surface of the loops of the substrate-binding domain of FBXW11, and the variants are predicted to destabilize the protein and/or its interactions. In situ hybridization studies on human and zebrafish embryonic tissues demonstrate FBXW11 is expressed in the developing eye, brain, mandibular processes, and limb buds or pectoral fins. Knockdown of the zebrafish FBXW11 orthologs fbxw11a and fbxw11b resulted in embryos with smaller, misshapen, and underdeveloped eyes and abnormal jaw and pectoral fin development. Our findings support the role of FBXW11 in multiple developmental processes, including those involving the brain, eye, digits, and jaw.

Original languageEnglish
Pages (from-to)640-657
Number of pages18
JournalAmerican Journal of Human Genetics
Volume105
Issue number3
DOIs
Publication statusPublished - Sep 5 2019

Fingerprint

Phenotype
Jaw
Brain
Zebrafish
SKP Cullin F-Box Protein Ligases
F-Box Proteins
Noonan Syndrome
Exome
Limb Buds
Catenins
Ubiquitination
Ligases
Ubiquitin
In Situ Hybridization
Proteins
Transcription Factors
Embryonic Structures
Genome
Databases

Keywords

  • brain
  • development
  • digit
  • eye
  • FBXW11
  • hedgehog
  • neurodevelopment
  • Noonan syndrome
  • WD40
  • Wnt

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

De Novo Missense Variants in FBXW11 Cause Diverse Developmental Phenotypes Including Brain, Eye, and Digit Anomalies. / Holt, Richard J.; Young, Rodrigo M.; Crespo, Berta; Ceroni, Fabiola; Curry, Cynthia J.; Bellacchio, Emanuele; Bax, Dorine A.; Ciolfi, Andrea; Simon, Marleen; Fagerberg, Christina R.; van Binsbergen, Ellen; De Luca, Alessandro; Memo, Luigi; Dobyns, William B.; Mohammed, Alaa Afif; Clokie, Samuel J.H.; Zazo Seco, Celia; Jiang, Yong Hui; Sørensen, Kristina P.; Andersen, Helle; Sullivan, Jennifer; Powis, Zöe; Chassevent, Anna; Smith-Hicks, Constance; Petrovski, Slavé; Antoniadi, Thalia; Shashi, Vandana; Gelb, Bruce D.; Wilson, Stephen W.; Gerrelli, Dianne; Tartaglia, Marco; Chassaing, Nicolas; Calvas, Patrick; Ragge, Nicola K.

In: American Journal of Human Genetics, Vol. 105, No. 3, 05.09.2019, p. 640-657.

Research output: Contribution to journalArticle

Holt, RJ, Young, RM, Crespo, B, Ceroni, F, Curry, CJ, Bellacchio, E, Bax, DA, Ciolfi, A, Simon, M, Fagerberg, CR, van Binsbergen, E, De Luca, A, Memo, L, Dobyns, WB, Mohammed, AA, Clokie, SJH, Zazo Seco, C, Jiang, YH, Sørensen, KP, Andersen, H, Sullivan, J, Powis, Z, Chassevent, A, Smith-Hicks, C, Petrovski, S, Antoniadi, T, Shashi, V, Gelb, BD, Wilson, SW, Gerrelli, D, Tartaglia, M, Chassaing, N, Calvas, P & Ragge, NK 2019, 'De Novo Missense Variants in FBXW11 Cause Diverse Developmental Phenotypes Including Brain, Eye, and Digit Anomalies', American Journal of Human Genetics, vol. 105, no. 3, pp. 640-657. https://doi.org/10.1016/j.ajhg.2019.07.005
Holt, Richard J. ; Young, Rodrigo M. ; Crespo, Berta ; Ceroni, Fabiola ; Curry, Cynthia J. ; Bellacchio, Emanuele ; Bax, Dorine A. ; Ciolfi, Andrea ; Simon, Marleen ; Fagerberg, Christina R. ; van Binsbergen, Ellen ; De Luca, Alessandro ; Memo, Luigi ; Dobyns, William B. ; Mohammed, Alaa Afif ; Clokie, Samuel J.H. ; Zazo Seco, Celia ; Jiang, Yong Hui ; Sørensen, Kristina P. ; Andersen, Helle ; Sullivan, Jennifer ; Powis, Zöe ; Chassevent, Anna ; Smith-Hicks, Constance ; Petrovski, Slavé ; Antoniadi, Thalia ; Shashi, Vandana ; Gelb, Bruce D. ; Wilson, Stephen W. ; Gerrelli, Dianne ; Tartaglia, Marco ; Chassaing, Nicolas ; Calvas, Patrick ; Ragge, Nicola K. / De Novo Missense Variants in FBXW11 Cause Diverse Developmental Phenotypes Including Brain, Eye, and Digit Anomalies. In: American Journal of Human Genetics. 2019 ; Vol. 105, No. 3. pp. 640-657.
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T1 - De Novo Missense Variants in FBXW11 Cause Diverse Developmental Phenotypes Including Brain, Eye, and Digit Anomalies

AU - Holt, Richard J.

AU - Young, Rodrigo M.

AU - Crespo, Berta

AU - Ceroni, Fabiola

AU - Curry, Cynthia J.

AU - Bellacchio, Emanuele

AU - Bax, Dorine A.

AU - Ciolfi, Andrea

AU - Simon, Marleen

AU - Fagerberg, Christina R.

AU - van Binsbergen, Ellen

AU - De Luca, Alessandro

AU - Memo, Luigi

AU - Dobyns, William B.

AU - Mohammed, Alaa Afif

AU - Clokie, Samuel J.H.

AU - Zazo Seco, Celia

AU - Jiang, Yong Hui

AU - Sørensen, Kristina P.

AU - Andersen, Helle

AU - Sullivan, Jennifer

AU - Powis, Zöe

AU - Chassevent, Anna

AU - Smith-Hicks, Constance

AU - Petrovski, Slavé

AU - Antoniadi, Thalia

AU - Shashi, Vandana

AU - Gelb, Bruce D.

AU - Wilson, Stephen W.

AU - Gerrelli, Dianne

AU - Tartaglia, Marco

AU - Chassaing, Nicolas

AU - Calvas, Patrick

AU - Ragge, Nicola K.

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