TY - JOUR
T1 - Deafferentation induces early and delayed differential changes in the pattern of expression of the various guanine nucleotide binding protein mRNAs in rat striatum
AU - Valerio, A.
AU - Tinti, C.
AU - Alberici, A.
AU - Belloni, M.
AU - Buonamici, M.
AU - Spano, P. F.
AU - Memo, M.
PY - 1993/12/24
Y1 - 1993/12/24
N2 - A polymerase chain reaction-derived method was used to identify and quantitate the relative abundance of the different mRNAs encoding various isoforms of the guanine nucleotide regulatory protein Gs, Gi, and Go α subunits in the striata of rats unilaterally injected with 6-hydroxydopamine in the substantia nigra. Thirty days after the lesion, the mRNA levels of the Go and of the Gi 1 α subunits were increased by about 2-3 times, those of the Gi 3 decreased by about 60% and those of Gi 2 and Gs unmodified. The pattern of expression of the Go α subunits mRNA changed in a time-dependent fashion, being significant 20 days after the lesion. The decrease in Gi 3 α subunit mRNA levels was maximum 10 days after the lesion and tended to be reduced in magnitude with time while the changes in Gi 1 α subunit mRNA showed a byphasic behaviour being reduced at 10 days and increased at 30 days after the lesion. These data suggest that the expression of the various G protein α subunits in the striatum are continuously regulated by factors originating from afferent neurons and surrounding cells.
AB - A polymerase chain reaction-derived method was used to identify and quantitate the relative abundance of the different mRNAs encoding various isoforms of the guanine nucleotide regulatory protein Gs, Gi, and Go α subunits in the striata of rats unilaterally injected with 6-hydroxydopamine in the substantia nigra. Thirty days after the lesion, the mRNA levels of the Go and of the Gi 1 α subunits were increased by about 2-3 times, those of the Gi 3 decreased by about 60% and those of Gi 2 and Gs unmodified. The pattern of expression of the Go α subunits mRNA changed in a time-dependent fashion, being significant 20 days after the lesion. The decrease in Gi 3 α subunit mRNA levels was maximum 10 days after the lesion and tended to be reduced in magnitude with time while the changes in Gi 1 α subunit mRNA showed a byphasic behaviour being reduced at 10 days and increased at 30 days after the lesion. These data suggest that the expression of the various G protein α subunits in the striatum are continuously regulated by factors originating from afferent neurons and surrounding cells.
KW - 6-Hydroxydopamine
KW - G protein
KW - Polymerase chain reaction
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U2 - 10.1016/0304-3940(93)90869-M
DO - 10.1016/0304-3940(93)90869-M
M3 - Article
C2 - 7512241
AN - SCOPUS:0027772307
VL - 164
SP - 109
EP - 112
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1-2
ER -