Deafferentation induces early and delayed differential changes in the pattern of expression of the various guanine nucleotide binding protein mRNAs in rat striatum

A. Valerio, C. Tinti, A. Alberici, M. Belloni, M. Buonamici, P. F. Spano, M. Memo

Research output: Contribution to journal › Article

Abstract

A polymerase chain reaction-derived method was used to identify and quantitate the relative abundance of the different mRNAs encoding various isoforms of the guanine nucleotide regulatory protein Gs, Gi, and Go α subunits in the striata of rats unilaterally injected with 6-hydroxydopamine in the substantia nigra. Thirty days after the lesion, the mRNA levels of the Go and of the Gi 1 α subunits were increased by about 2-3 times, those of the Gi 3 decreased by about 60% and those of Gi 2 and Gs unmodified. The pattern of expression of the Go α subunits mRNA changed in a time-dependent fashion, being significant 20 days after the lesion. The decrease in Gi 3 α subunit mRNA levels was maximum 10 days after the lesion and tended to be reduced in magnitude with time while the changes in Gi 1 α subunit mRNA showed a byphasic behaviour being reduced at 10 days and increased at 30 days after the lesion. These data suggest that the expression of the various G protein α subunits in the striatum are continuously regulated by factors originating from afferent neurons and surrounding cells.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalNeuroscience Letters
Volume164
Issue number1-2
DOIs
Publication statusPublished - Dec 24 1993

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Guanine Nucleotides
Carrier Proteins
Messenger RNA
GTP-Binding Proteins
Afferent Neurons
Oxidopamine
Protein Subunits
Substantia Nigra
Protein Isoforms
Polymerase Chain Reaction

Keywords

  • 6-Hydroxydopamine
  • G protein
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Deafferentation induces early and delayed differential changes in the pattern of expression of the various guanine nucleotide binding protein mRNAs in rat striatum. / Valerio, A.; Tinti, C.; Alberici, A.; Belloni, M.; Buonamici, M.; Spano, P. F.; Memo, M.

In: Neuroscience Letters, Vol. 164, No. 1-2, 24.12.1993, p. 109-112.

Research output: Contribution to journal › Article

Valerio, A, Tinti, C, Alberici, A, Belloni, M, Buonamici, M, Spano, PF & Memo, M 1993, 'Deafferentation induces early and delayed differential changes in the pattern of expression of the various guanine nucleotide binding protein mRNAs in rat striatum', Neuroscience Letters, vol. 164, no. 1-2, pp. 109-112. https://doi.org/10.1016/0304-3940(93)90869-M
Valerio A, Tinti C, Alberici A, Belloni M, Buonamici M, Spano PF et al. Deafferentation induces early and delayed differential changes in the pattern of expression of the various guanine nucleotide binding protein mRNAs in rat striatum. Neuroscience Letters. 1993 Dec 24;164(1-2):109-112. https://doi.org/10.1016/0304-3940(93)90869-M
Valerio, A. ; Tinti, C. ; Alberici, A. ; Belloni, M. ; Buonamici, M. ; Spano, P. F. ; Memo, M. / Deafferentation induces early and delayed differential changes in the pattern of expression of the various guanine nucleotide binding protein mRNAs in rat striatum. In: Neuroscience Letters. 1993 ; Vol. 164, No. 1-2. pp. 109-112.
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