Deafferentation up-regulates the expression of the mGlu1a metabotropic glutamate receptor protein in the olfactory bulb

G. Casabona, M. V. Catania, M. Storto, N. Ferraris, I. Perroteau', A. Fasolo, F. Nicoletti, P. Bovolin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chemical lesion of olfactory neuroepitheium induced an up-regulation of the mGlu1a metabotropic glutamate receptor protein in the olfactory bulb, as shown by Western blot analysis. At 2 days after the lesion, the increase in the receptor protein was associated with an increase in mGlu1a mRNA levels; in contrast, at longer times after the lesion (16 days), mRNA levels were reduced in spite of the high expression of the receptor protein, perhaps as a result of product-inhibition of mGlu1 gene expression. Immunohistochemical analysis showed that the increase in mGlu1a induced by olfactory denervation was confined to the glomeruli, which occupy the external portion of the olfactory bulb. Within these structures, mGlu1a receptors are mainly localized on the distal dendrites of mitral cells, which are innervated by the glutamatergic axons of the olfactory nerve, These results demonstrate that the expression of mGlu1a receptors is up-regulated in response to glutamatergic deafferentation, supporting a role for this particular receptor subtype in the physiology of synaptic transmission.

Original languageEnglish
Pages (from-to)771-776
Number of pages6
JournalEuropean Journal of Neuroscience
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 1998

Fingerprint

Metabotropic Glutamate Receptors
Olfactory Bulb
Up-Regulation
Olfactory Nerve
Messenger RNA
Proteins
Denervation
Dendrites
Synaptic Transmission
Axons
Western Blotting
Gene Expression

Keywords

  • Deafferentation
  • mGlu1a
  • Olfactory bulb

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Deafferentation up-regulates the expression of the mGlu1a metabotropic glutamate receptor protein in the olfactory bulb. / Casabona, G.; Catania, M. V.; Storto, M.; Ferraris, N.; Perroteau', I.; Fasolo, A.; Nicoletti, F.; Bovolin, P.

In: European Journal of Neuroscience, Vol. 10, No. 2, 02.1998, p. 771-776.

Research output: Contribution to journalArticle

Casabona, G. ; Catania, M. V. ; Storto, M. ; Ferraris, N. ; Perroteau', I. ; Fasolo, A. ; Nicoletti, F. ; Bovolin, P. / Deafferentation up-regulates the expression of the mGlu1a metabotropic glutamate receptor protein in the olfactory bulb. In: European Journal of Neuroscience. 1998 ; Vol. 10, No. 2. pp. 771-776.
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AU - Nicoletti, F.

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