Decidual natural killer cell tuning by autologous dendritic cells

Gordana Laškarin, Arnela Redžović, Željka Rubeša, Alberto Mantovani, Paola Allavena, Herman Haller, Ivan Vlastelić, Daniel Rukavina

Research output: Contribution to journalArticlepeer-review

Abstract

Problem: Dendritic cells (DC)/natural killer (NK) cells interactions in the deciduas of early human pregnancies were analyzed in vitro. Method of Study: Phenotype, cytokine expression and/or cytolytic mediators' expression were measured by flow cytometry in NK and DC from the freshly isolated decidual mononuclear cells or after their purification and co-culture in vitro. Proliferation of 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE)-labeled CD56+ cells was analyzed by flow cytometry after the co-culture with CD1a+ or CD83+ DC. Results: Decidual CD1a+ cells show less mature phenotype with no expression of CD197, lower expression of CD80 and CD86 and higher expression of CD206 and CD195 in comparison to CD83+ cells. Interleukin (IL)-15, interferon-γ and tumor necrosis factor-α productions were higher in immature than mature DC, whereas IL-10 and IL-18 were equally produced in both subpopulations. Immature DC increase perforin, FasL and TRAIL protein expression and proliferation of NK cells, but decrease their intracellular IL-15 production. Mature DC caused less efficient proliferation of NK cells, and did not affect cytokine and cytolytic mediator expression. Conclusion: These results suggest that decidual CD1a+ cells regulate and shape NK cell function more profoundly than CD83+ cells in decidua.

Original languageEnglish
Pages (from-to)433-445
Number of pages13
JournalAmerican Journal of Reproductive Immunology
Volume59
Issue number5
DOIs
Publication statusPublished - May 2008

Keywords

  • Cytokine
  • Cytolytic mediators
  • DC/NK interaction
  • Decidua
  • NK proliferation pregnancy

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

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