Decrease in mitochondrial energy coupling by thyroid hormones: A physiological effect rather than a pathological hyperthyroidism consequence

V. Bobyleva, T. L. Pazienza, R. Maseroli, A. Tomasi, S. Salvioli, A. Cossarizza, C. Franceschi, V. P. Skulachev

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of the in vivo thyroid status on mitochondrial membrane potential (ΔΨ(m)) in isolated rat hepatocytes was studies by means of a cytofluorimetric technique and the ΔΨ(m)-specific probe JC-1. It is shown that the ΔΨ(m) level decreases in the order hypothyroid > euthyroid > hyperthyroid. Polarographic measurement of the hepatocyte respiratory rates revealed an opposite trend of values: the highest respiratory rate in hepatocytes from hyperthyroid animals, the lowest in those from hypothyroid ones. This means that mitochondrial energy coupling is highest in hypothyroid hepatocytes and lowest in hyperthyroid hepatocytes. 6-Ketocholestanol added to hepatocytes failed to counterbalance the uncoupling effect of thyroid hormones on ΔΨ(m) and respiration rate. Under the same conditions, 6- ketocholestanol appeared to be effective in recoupling of respiration uncoupled by low concentrations of the artificial protonophore FCCP. The mechanism and possible physiological functions of the thyroid hormone- induced decrease in mitochondrial energy coupling are discussed.

Original languageEnglish
Pages (from-to)409-413
Number of pages5
JournalFEBS Letters
Volume430
Issue number3
DOIs
Publication statusPublished - Jul 3 1998

Keywords

  • Hepatocyte
  • Membrane potential
  • Mitochondrion
  • Thyroid hormone
  • Uncoupling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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